Teachable, high-content analytics for live-cell, phase contrast movies.

Journal of Biomolecular Screening
Samuel V AlworthJames S J Lee

Abstract

CL-Quant is a new solution platform for broad, high-content, live-cell image analysis. Powered by novel machine learning technologies and teach-by-example interfaces, CL-Quant provides a platform for the rapid development and application of scalable, high-performance, and fully automated analytics for a broad range of live-cell microscopy imaging applications, including label-free phase contrast imaging. The authors used CL-Quant to teach off-the-shelf universal analytics, called standard recipes, for cell proliferation, wound healing, cell counting, and cell motility assays using phase contrast movies collected on the BioStation CT and BioStation IM platforms. Similar to application modules, standard recipes are intended to work robustly across a wide range of imaging conditions without requiring customization by the end user. The authors validated the performance of the standard recipes by comparing their performance with truth created manually, or by custom analytics optimized for each individual movie (and therefore yielding the best possible result for the image), and validated by independent review. The validation data show that the standard recipes' performance is comparable with the validated truth with low variation. T...Continue Reading

References

Jul 12, 2003·Journal of Biomolecular Screening·Peter RammBohdan J Soltys
Dec 31, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joakim LindbladAlla Zaltsman
Nov 13, 2004·Science·Zachary E PerlmanSteven J Altschuler
Jul 14, 2005·Journal of Biomedical Informatics·Xiaobo ZhouStephen T C Wong
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Dec 11, 2008·The Journal of Pharmacology and Experimental Therapeutics·Marni Brisson TiernoJohn S Lazo
Jan 14, 2009·Molecular and Cellular Biology·Rosanna Pescini GobertRob Hooft van Huijsduijnen
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Methods Mentioned

BETA
fluorescence imaging
motility tracking
motility measurements

Software Mentioned

Quant
CL

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