Tear Film Proteomics Reveal Important Differences Between Patients With and Without Ocular GvHD After Allogeneic Hematopoietic Cell Transplantation

Investigative Ophthalmology & Visual Science
Nadine Gerber-HollbachDavid Goldblum

Abstract

To date, no biomarkers for ocular graft versus host disease (GvHD), a frequent complication following allogeneic hematopoietic cell transplantation (HCT), exist. In this prospective study, we evaluated the potential of human tear proteins as biomarkers for ocular GvHD. Tears from 10 patients with moderate-to-severe ocular GvHD were compared to 10 patients without ocular GvHD. After a full ocular surface clinical examination, tears were collected onto Schirmer strips and protein composition was analyzed by liquid chromatography tandem mass spectrometry. Statistical evaluation was performed using the Mann-Whitney U test to compare means and the false discovery rate method to adjust for multiple comparisons. Functional annotation of differentially expressed proteins was done with the PANTHER classification system. We identified 282 proteins in tryptic digests of Schirmer strips; 79 proteins were significantly differentially expressed between the two groups, from which 54 were up- and 25 downregulated. The most upregulated proteins were classified as nucleic acid binding and cytoskeletal proteins, while the most extensively downregulated proteins belong to an array of classes including transfer and receptor proteins, enzyme modulat...Continue Reading

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Citations

Sep 4, 2020·Translational Vision Science & Technology·Olivia E O'LearyDavid Goldblum
Oct 2, 2019·Taiwan Journal of Ophthalmology·Pak Yui FongLouis Tong
Oct 16, 2018·Experimental Eye Research·Marianne DorNatacha Turck
Apr 30, 2021·Indian Journal of Ophthalmology·Sridevi NairYoko Ogawa
Jul 3, 2021·International Journal of Molecular Sciences·Yoko OgawaKazuo Tsubota
Nov 10, 2021·Acta Ophthalmologica·Janika NättinenHannu Uusitalo

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