Tear fluid content of electrochemically active components including water soluble antioxidants

Current Eye Research
R GogiaR C Rose

Abstract

To describe the content of water-soluble antioxidants in tear fluid. We collected tear fluid from healthy subjects, either into borosilicate glass tubing or by absorption onto Schirmer strips. High pressure liquid chromatography with electrochemical detection provided data on several components in a single assay. The system was sufficiently sensitive to provide reliable values for components present in tear fluid collected at normal (basal) or stimulated rates of secretion. Tear fluid at basal secretion was found to contain four compounds often considered to function in biology as antioxidants. Ascorbic acid (AA) is found at 665 microM, tyrosine at 45 microM and glutathione (GSH) at 107 microM. Cysteine (48 microM) and uric acid (328 microM) are reported for the first time; the latter is somewhat controversial as a physiologically active antioxidant. One peak on the chromatogram was consistently present but has not been identified. During stimulation of flow by brief inhalation of ammonium hydroxide fumes, the concentration of each compound was lower. This work demonstrates the presence of electro-chemically active components that might function as antioxidants at the anterior surface of the cornea against potential damage from...Continue Reading

References

Jan 1, 1986·Journal of Ocular Pharmacology·C H BonneyD Fong
May 1, 1986·Archives of Ophthalmology·G R ReissR F Brubaker
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B N AmesP Hochstein
Sep 1, 1980·Ophthalmology·A Jordan, J Baum
Mar 1, 1995·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·F M Bonilla-MusolesA Pellicer
Feb 15, 1995·The Biochemical Journal·R C Rose, A M Bode
Jul 1, 1993·Current Eye Research·A M BodeR C Rose
Nov 19, 1993·Brain Research·R C Rose
Jun 1, 1993·Free Radical Biology & Medicine·B F Becker
Feb 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·R Dreyer, R C Rose
Jan 1, 1995·Lasers in Surgery and Medicine·S JainD T Azar

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Citations

Dec 24, 2004·The British Journal of Ophthalmology·J Horwath-WinterG Rieger
Nov 26, 2002·The British Journal of Ophthalmology·V PeponisN M Sitaras
Jan 11, 2011·Expert Opinion on Pharmacotherapy·Murat Dogru, Kazuo Tsubota
Sep 11, 2012·Experimental Eye Research·Aluru Venkata SaijyothiNarayanasamy Angayarkanni
Jun 27, 2012·Progress in Retinal and Eye Research·Lei Zhou, Roger W Beuerman
Feb 10, 2012·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Amandeep Panaser, Brian J Tighe
Oct 17, 2007·The Ocular Surface·Hovhannes J GukasyanRam Kannan
Aug 23, 2008·Free Radical Biology & Medicine·Akiva ShohamHyman M Schipper
Dec 14, 2007·Seminars in Cell & Developmental Biology·Natalie LassenVasilis Vasiliou
Jan 15, 2004·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Camus Kar Man ChoyPauline Cho
Jun 13, 2008·Acta Ophthalmologica·Jutta Horwath-WinterOtto Schmut
Dec 22, 2009·Biomedical Chromatography : BMC·Constantine D GeorgakopoulosNikos K Karamanos
Nov 13, 2015·Archivos de la Sociedad Española de Oftalmología·A M Muñoz-HernándezJ M Benítez-del-Castillo
Aug 2, 2011·Journal of Proteome Research·Liyan ChenRoger W Beuerman
Jun 6, 2013·Analytical Chemistry·Magnus FalkSergey Shleev
Feb 17, 2017·ACS Applied Materials & Interfaces·Muhammad S KhanDipanjan Pan
Sep 23, 2003·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Camus Kar Man ChoyIris F F Benzie
May 25, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Gebhard RiegerBirgit Spitzer-Sonnleitner
Feb 9, 2013·Analytical and Bioanalytical Chemistry·Viktor AndoralovTautgirdas Ruzgas
Apr 28, 2009·European Journal of Ophthalmology·Sophie Drouault-HolowaczFrédéric Chiambaretta
Apr 11, 2020·Oxidative Medicine and Cellular Longevity·Ming-Xuan WangYa-Juan Zheng
Mar 6, 2017·Biochemistry. Biokhimii︠a︡·E Yu ZerniiI I Senin
Aug 4, 2019·International Journal of Molecular Sciences·Mazyar YazdaniTor Paaske Utheim
May 13, 2021·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Eleonora QuartieriThelma A Pertinhez
Dec 2, 2009·The Ocular Surface·Ying ChenVasilis Vasiliou

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