PMID: 8575611Mar 1, 1995Paper

Tear malate dehydrogenase, lactate dehydrogenase and their isoenzymes in normal Chinese subjects and patients of ocular surface disorders

Yan ke xue bao = Eye science
Q Guo, H C Zhang

Abstract

To determine levels of malate dehydrogenase (MDH), lactate dehydrogenase (LDH) and their isoenzymes in tears of normal Chinese subjects and patients with ocular surface disorders. The age range of normal subjects was 10-88, with 136 male and 128 female subjects. 123 patients suffered from ocular surface disorders. Tears were collected from lower fornix on Xinghua filter disc (0.1mm thick, 5mm in diameter). The values of tear MDH and LDH were determined by MONARCH-2000 Analyzer (U. S. A.). Their isoenzymes were separated by acetate cellulose electrophoresis and were determined by Model CDS-200 light densitometer. The normal values of tear LDH and MDH were 45.51 + 23.00-81.35 + 37.84 umol.s-1/L and 11.00 + 5.33-19.50 + 9.17 umol.s-1/L respectively, disregarding sex or eye distriction (P > 0.05). The values of tear LDH and MDH in the group aged 10-19 were significantly lower than in another groups (P < 0.05). 95% normal ranges of tear MDH aged below 19 and above 20 were 3.63-19.90 umol.s-1/L and 4.20-36.64 umol s-1/L. 95% normal ranges of tear LDH aged below 19 and above 20 were 17.69-82.93 umol.s-1/L and 21.47-150.41 umol.s-1/L. The MDH isoenzymes comprised MDHs and MDHm, the former accounting for 80.0-89.1%. The LDH isoenzymes c...Continue Reading

Related Concepts

Anterior Chamber Endothelium
Alloenzymes
Keratoconjunctivitis
Lactate Dehydrogenase
Malate Dehydrogenase
Tears Body Substance
Trachoma
Keratitis, Herpetic

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