PMID: 15240663Jul 9, 2004Paper

Tec kinase migrates to the T cell-APC interface independently of its pleckstrin homology domain

The Journal of Immunology : Official Journal of the American Association of Immunologists
Fabien GarçonJacques A Nunès

Abstract

Tec is the prototypical member of the Tec tyrosine kinases family, which plays an important role in T cell signaling. We show in this study that Tec translocates to the immunological synapse when a T cell contacts a dendritic cell. Surprisingly, the presence of the pleckstrin homology (PH) domain of Tec is not required for this accumulation, and despite a strong activation of 3'-phosphorylated phosphoinositide lipids synthesis during the synapse formation, the Tec PH domain is not redistributed to the T cell plasma membrane. In contrast, we demonstrate that an active Src homology 3 domain is absolutely required, underlining the essential role played by this part of the molecule in the recruitment and/or stabilization of Tec at the immunological synapse. Our results nevertheless suggest that the PH domain controls the kinase activity of the molecule in vivo. We finally demonstrate that the two domains are necessary to trigger transcriptional events following Ag presentation. These data support a model in which the plasma membrane recruitment of the PH-containing protein Tec is not dependent on the production of 3'-phosphorylated phosphoinositide lipids by the PI3K, but rather on an intact Src homology 3 domain.

References

Jul 16, 1993·Cell·D Bar-SagiJ Schlessinger
Oct 11, 1996·The Journal of Biological Chemistry·S C BunnellL J Berg
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·A AugustH Hanafusa
Jan 5, 1999·The Journal of Biological Chemistry·W C YangD Olive
Jan 15, 2000·The Journal of Biological Chemistry·S C BunnellL J Berg
Apr 27, 2000·Current Opinion in Immunology·E M Schaeffer, P L Schwartzberg
May 5, 2000·Immunity·W C YangD Olive
Sep 13, 2000·Molecular Cell·S E LietzkeD G Lambright
Nov 4, 2000·International Immunology·W C YangD Olive
Dec 6, 2000·The Journal of Experimental Medicine·W EllmeierD R Littman
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W C YangL J Berg
Feb 23, 2002·Science·Kyeong-Hee LeeAndrey S Shaw
Apr 26, 2002·Current Opinion in Immunology·Andrew T Miller, Leslie J Berg
Oct 22, 2002·Nature Immunology·Julie Harriague, Georges Bismuth
Oct 22, 2002·Nature Immunology·Patrick S CostelloDoreen A Cantrell
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juris A GrasisConstantine D Tsoukas
Jul 5, 2003·The Journal of Biological Chemistry·Heather M Wilcox, Leslie J Berg
Mar 3, 2004·Molecular and Cellular Biology·Michael G TomlinsonArthur Weiss

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Citations

Jul 13, 2007·Immunological Reviews·Julio Gomez-RodriguezPamela L Schwartzberg
Mar 18, 2009·Immunological Reviews·David A Fruman, Georges Bismuth
Apr 2, 2005·Nature Reviews. Immunology·Pamela L SchwartzbergJulie A Readinger
Mar 18, 2005·Annual Review of Immunology·Leslie J BergPamela L Schwartzberg
Oct 20, 2004·The Journal of Biological Chemistry·Michael G TomlinsonArthur Weiss
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Audrey GérardJacques A Nunès
Jun 10, 2009·Inflammation·Guang-Qing WangZhao-Fan Xia
Jul 15, 2011·Molecular Immunology·Nassima MessalDaniel Olive

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