PMID: 8971860Oct 1, 1996Paper

Technetium-99m antibodies labeled with MAG3 and SHNH: an in vitro and animal in vivo comparison

Nuclear Medicine and Biology
K LeiD J Hnatowich

Abstract

The in vitro stability and animal pharmacokinetics of 99mTc bound to Sandoz and C110 IgG antibodies via a modified MAG3 has been compared with the hydrazino nicotinamide (SHNH) moiety as standard. For both antibodies, the stabilities of the label to challenge at up to 50:1 cysteine: IgG molar ratio were comparable, but at higher molar ratios, MAG3 showed greater instabilities. For the Sandoz antibody, size-exclusion HPLC analysis of 37 degrees C serum incubates and plasma samples from injected mice showed no clearly distinguishable differences. In the C110 case, some increased high molecular weight radioactivity was apparent with MAG3. Biodistributions in normal mice showed significant differences only in liver (Sandoz) and liver, spleen, intestines, stomach, and blood (C110), with SHNH usually providing higher levels. Thus, for two different antibodies and under the conditions of this study, the MAG3 chelator provided a 99mTc label with properties similar to that of SHNH moiety.

References

Nov 1, 1995·Nuclear Medicine Communications·K VerbekeA Verbruggen
Nov 1, 1994·Nuclear Medicine and Biology·D J HnatowichP Winnard
Jul 1, 1997·Nuclear Medicine and Biology·P WinnardD J Hnatowich

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Citations

Dec 6, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Torun EngfeldtVladimir Tolmachev
Sep 23, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Anna OrlovaVladimir Tolmachev
May 1, 1997·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·D J HnatowichM Rusckowski
Aug 24, 2001·Nuclear Medicine and Biology·Z ZhuD J Hnatowich
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alan J BitontiRichard S Blumberg
Jul 13, 2004·Nuclear Medicine and Biology·Dik BlokJan W Drijfhout
Jul 1, 1997·Nuclear Medicine and Biology·P WinnardD J Hnatowich
Apr 5, 2007·Nature Protocols·Yi WangDonald J Hnatowich
Dec 24, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·M RusckowskiD J Hnatowich
Mar 14, 2009·Chemical Communications : Chem Comm·Mark BartholomäJon Zubieta
Feb 26, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·João D G CorreiaIsabel Santos
Dec 26, 2001·Chemical Reviews·S Liu, D S Edwards
Jan 12, 2011·Nuclear Medicine and Biology·Rodrigo TeodoroRodrigo Guimarães Queiróz

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