Technetium-99m-labeled white blood cells: a new method to define the local and systemic role of leukocytes in acute experimental pancreatitis

Annals of Surgery
J WernerA L Warshaw

Abstract

We developed a new method to quantitate leukocyte accumulation in tissues and used it to examine the time course and severity of acute experimental pancreatitis. Leukocyte activation and infiltration are believed to be critical steps in the progression from mild to severe pancreatitis and responsible for many of its systemic complications. Pancreatitis of graded severity was induced in Sprague-Dawley rats with a combination of caerulein and controlled intraductal infusion. Technetium-99m (99mTc)-labeled leukocytes were quantified in pancreas, lung, liver, spleen, and kidney and compared with myeloperoxidase activity. The severity of pancreatitis was ascertained by wet/dry weight ratio, plasma amylase, and trypsinogen activation peptide in the pancreas. The time course of leukocyte accumulation was determined over 24 hours. Pancreatic leukocyte infiltration correlated well with tissue myeloperoxidase concentrations. In mild pancreatitis, leukocytes accumulated only in the pancreas. Moderate and severe pancreatitis were characterized by much greater leukocyte infiltration in the pancreas than in mild disease (p < 0.01), and increased 99mTc radioactivity was detectable in the lung as early as 3 hours. 99mTc radioactivity correlate...Continue Reading

References

Jan 1, 1992·Annals of Surgery·J SchmidtA L Warshaw
Jan 1, 1989·Virchows Archiv. A, Pathological Anatomy and Histopathology·S WillemerG Adler
Oct 1, 1987·British Journal of Diseases of the Chest·G S BasranR Verma
Jan 1, 1988·Pancreas·C S RobertsonJ G Hardy
Jul 1, 1988·Annals of Surgery·K S GuiceP A Ward
Jul 22, 1988·Journal of Immunological Methods·P R HurleyJ Hermon-Taylor
Nov 1, 1987·Microvascular Research·S D House, H H Lipowsky
Dec 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·M CeskaB Brown
Mar 1, 1982·The Journal of Investigative Dermatology·P P BradleyG Rothstein
Jan 1, 1984·Methods in Enzymology·S J KlebanoffH Rosen
Dec 1, 1981·The Journal of Clinical Investigation·J M HarlanD G Wright
Jan 1, 1980·Microvascular Research·G W Schmid-SchönbeinS Chien
Nov 1, 1995·American Journal of Respiratory and Critical Care Medicine·P MontraversJ M Desmonts
Mar 1, 1994·The American Journal of Physiology·H Simms, R D'Amica
Jan 1, 1995·Biochemical Society Symposium·J P Uetrecht

❮ Previous
Next ❯

Citations

Sep 24, 1999·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·E AlhanS Al
Aug 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Werner HartwigMarkus W Büchler
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E VaqueroS J Pandol
Apr 26, 2006·Scandinavian Journal of Gastroenterology·Changbin ShiRoland Andersson
Dec 10, 2013·Critical Care : the Official Journal of the Critical Care Forum·Kai A BachmannOliver Mann
Apr 9, 2001·Pancreas·A Szuster-CiesielskaM Kandefer-Szerszeń
Oct 20, 2000·Pancreas·W HartwigE Klar
Oct 25, 2011·Journal of Translational Medicine·Argyris TzouvelekisDemosthenes Bouros
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jens WernerCarlos Fernández-Del Castillo
Nov 24, 1999·The American Journal of Physiology·W HartwigC Fernández-del Castillo
Oct 30, 2020·Drug Delivery·Xue JiangLongfa Kou
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·M C ManjabacasR Varón
Oct 4, 2007·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Marco Del ChiaroFranco Mosca
Sep 21, 2010·Life Sciences·Thilo HackertJens Werner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
T HiranoK Endo
Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
H L AtkinsL R Chervu
Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
S Vallabhajosula
Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
M W Billinghurst, B A Westendorf
© 2021 Meta ULC. All rights reserved