Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties.

Journal of Leukocyte Biology
Penelope ZorziRoberto F Nicosia

Abstract

Angiogenesis in the aortic ring model is preceded by activation of the immune system and impaired by ablation of adventitial macrophages. Treatment of aortic cultures with M-CSF induced extensive periaortic outgrowth of CD45(+) CD68(+) mononuclear cells with ultrastructural features of macrophages and DCs. Periaortic lysis of collagen caused many CD45(+) CD68(+) cells to attach to the bottom of the culture dish. Lifting the collagen gels left behind patches of CD45(+) CD68(+) cells, which focally organized into branching cords. These cells also expressed CD14, CD169, F4/80, and α-SMA but not CD31, vWF, desmin, or CD163. DNA synthesis studies showed that M-CSF-stimulated cells were actively proliferating. Aortic patch cells showed phagocytic properties and responded to IL-4 and GM-CSF by expressing MHC II, differentiating into DCs, and forming multinucleated giant cells. They also stimulated angiogenesis and VEGF production in aortic ring cultures. This study demonstrates that the rat aorta contains a distinct subset of immature immunocytes capable of proliferating, differentiating into macrophages and DCs, and stimulating angiogenesis. Isolation of these cells in patches from M-CSF-stimulated aortic rings provides a reproducibl...Continue Reading

References

Nov 1, 1992·Journal of Leukocyte Biology·H HondaA Rostami
Mar 1, 1994·Journal of Leukocyte Biology·C SunderkötterC Sorg
Jun 1, 1994·In Vitro Cellular & Developmental Biology. Animal·R F NicosiaM Smith
Feb 1, 1996·Journal of Leukocyte Biology·M NaitoK Takahashi
Apr 1, 1997·The Japanese Journal of Physiology·H FukaoO Matsuo
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Matthias HeilWolfgang Schaper
Dec 18, 2003·The Journal of Clinical Investigation·Stuart P WeisbergAnthony W Ferrante
Feb 3, 2005·Stem Cells and Development·Mirela AnghelinaNicanor I Moldovan
Mar 16, 2005·Journal of Vascular Research·Alexander JabsJosiah N Wilcox
Dec 16, 2005·Nature·Peter Carmeliet
Jan 21, 2006·Cancer Research·Claire E Lewis, Jeffrey W Pollard
May 11, 2006·Journal of Leukocyte Biology·Caroline E BergmannIvo R Buschmann
Feb 27, 2007·The American Journal of Pathology·Machteld J van AmerongenMarja J A van Luyn
Dec 19, 2007·Cancer Metastasis Reviews·Douglas M NoonanAdriana Albini
Feb 7, 2008·Pathology International·Makoto Naito
May 22, 2008·American Journal of Physiology. Endocrinology and Metabolism·Can PangJianping Ye
Jun 19, 2008·Kidney International·D Ferenbach, J Hughes
Sep 6, 2008·Methods in Enzymology·Alfred C AplinRoberto F Nicosia
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maurizio GelatiRoberto F Nicosia
Feb 10, 2009·Current Pharmaceutical Design·Zhao Ming David DongRoberto F Nicosia
Mar 17, 2009·Biochemical and Biophysical Research Communications·Kosuke AzumaHirotaka Watada
Aug 7, 2009·Autoimmunity·Kisha PiggottCornelia M Weyand

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Citations

Oct 19, 2011·Cells, Tissues, Organs·Mark W MajeskyWilliam M Mahoney
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Mark W MajeskyGuenter Daum
Mar 5, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Giovanni LigrestiRoberto F Nicosia
Dec 12, 2012·Annual Review of Physiology·Kurt R StenmarkMaria G Frid
Oct 21, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maki KabaraNaoyuki Hasebe
Aug 9, 2016·Pharmacology & Therapeutics·Philipp WörsdörferSüleyman Ergün
May 17, 2018·Angiogenesis·Patrycja Nowak-SliwinskaArjan W Griffioen
May 26, 2018·Circulation Research·Li ZhangQingbo Xu
Jul 13, 2014·Circulation·Michael Sze Ka WongRalf P Brandes
Apr 11, 2015·Circulation Research·Peter J Psaltis, Robert D Simari
Apr 11, 2019·Frontiers in Cardiovascular Medicine·Shahida ShafiSihem Aouabdi
May 26, 2017·Antioxidants & Redox Signaling·Baoqi YuQingbo Xu

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