Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.

Frontiers in Endocrinology
Jiri Pavlacky, Jan Polak

Abstract

Hypoxia is characterized as insufficient oxygen delivery to tissues and cells in the body and is prevalent in many human physiology processes and diseases. Thus, it is an attractive state to experimentally study to understand its inner mechanisms as well as to develop and test therapies against pathological conditions related to hypoxia. Animal models in vivo fail to recapitulate some of the key hallmarks of human physiology, which leads to human cell cultures; however, they are prone to bias, namely when pericellular oxygen concentration (partial pressure) does not respect oxygen dynamics in vivo. A search of the current literature on the topic revealed this was the case for many original studies pertaining to experimental models of hypoxia in vitro. Therefore, in this review, we present evidence mandating for the close control of oxygen levels in cell culture models of hypoxia. First, we discuss the basic physical laws required for understanding the oxygen dynamics in vitro, most notably the limited diffusion through a liquid medium that hampers the oxygenation of cells in conventional cultures. We then summarize up-to-date knowledge of techniques that help standardize the culture environment in a replicable fashion by increa...Continue Reading

References

Apr 10, 1965·Nature·K M Stevens
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
May 1, 1995·Respiration Physiology·E MetzenW Jelkmann
Apr 1, 1994·The American Journal of Physiology·W L RumseyD F Wilson
Feb 24, 2001·American Journal of Physiology. Cell Physiology·C M Otto, J E Baumgardner
Sep 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C B AllenC W White
Oct 26, 2002·Free Radical Biology & Medicine·Carine MichielsMartine Raes
Dec 14, 2002·American Journal of Respiratory and Critical Care Medicine·Yoshihisa MoritaClaudio M Martin
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria WartenbergHeinrich Sauer
Jul 11, 2003·Respiratory Physiology & Neurobiology·James E Baumgardner, Cynthia M Otto
Mar 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshihiko EzashiR Michael Roberts
Mar 15, 2006·Blood Cells, Molecules & Diseases·Neema Jamshidi, Bernhard Ø Palsson
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Armen Y MulkidjanianMichael Y Galperin
Nov 1, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·David ZagzagElizabeth W Newcomb
Jul 5, 2007·Pflügers Archiv : European journal of physiology·Bohan WangPaul Trayhurn
Oct 30, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Siranoush ShahrzadBrenda L Coomber
Nov 17, 2007·American Journal of Respiratory and Critical Care Medicine·Larissa DyugovskayaLena Lavie
Oct 15, 2008·Pediatric Research·Emin Maltepe, Ola Didrik Saugstad
Oct 17, 2008·Aviation, Space, and Environmental Medicine·Johnny Conkin, James H Wessel
Dec 4, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J Ye
Dec 17, 2008·The Kaohsiung Journal of Medical Sciences·Bi-Fang LeeLie-Hang Shen
Feb 3, 2009·Angiogenesis·Sébastien ToffoliCarine Michiels
Mar 7, 2009·Journal of Applied Physiology·Mariam Louis, Naresh M Punjabi
Mar 12, 2009·Vascular Pharmacology·Tamás RadovitsGábor Szabó
May 23, 2009·Cancer Science·Shinae Kizaka-Kondoh, Hideko Konse-Nagasawa
Sep 15, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kurt R StenmarkIvan F McMurtry
Sep 18, 2009·Angiogenesis·Sébastien ToffoliCarine Michiels
Dec 8, 2009·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Shawn C Oppegard, David T Eddington
Jul 27, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Alexander V ZhdanovDmitri B Papkovsky
Aug 5, 2010·Cell Stem Cell·Ahmed MohyeldinAlfredo Quiñones-Hinojosa
Dec 4, 2010·Nature Protocols·Ziquan CaoYihai Cao
Dec 24, 2010·American Journal of Physiology. Cell Physiology·Larissa A Shimoda, Jan Polak

❮ Previous
Next ❯

Citations

Feb 3, 2021·Cancers·Tord HomplandHeidi Lyng
Mar 7, 2021·International Journal of Molecular Sciences·Pauline ChazelasJérôme Guillard
Feb 4, 2021·Advanced Healthcare Materials·Jay R K SamalOommen P Oommen
Mar 23, 2021·Cellular and Molecular Life Sciences : CMLS·Jan SpaasTim Vanmierlo
Mar 23, 2021·Scientific Reports·Adriana M De MendozaDamian D McLeod
Jun 12, 2021·Cardiovascular Toxicology·Shafreena Shaukat AliWan Amir Nizam Wan Ahmad
Aug 3, 2021·Frontiers in Cardiovascular Medicine·Natalie N Khalil, Megan L McCain
Aug 28, 2021·Cells·Miriam Di MattiaBarbara Barboni

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
biopsies

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.