Technical feasibility study for production of tailored multielectrode arrays and patterning of arranged neuronal networks

PloS One
Matthias SchürmannAndy Thomas

Abstract

In this manuscript, we first reveal a simple ultra violet laser lithographic method to design and produce plain tailored multielectrode arrays. Secondly, we use the same lithographic setup for surface patterning to enable controlled attachment of primary neuronal cells and help neurite guidance. For multielectrode array production, we used flat borosilicate glass directly structured with the laser lithography system. The multi layered electrode system consists of a layer of titanium coated with a layer of di-titanium nitride. Finally, these electrodes are covered with silicon nitride for insulation. The quality of the custom made multielectrode arrays was investigated by light microscopy, electron microscopy and X-ray diffraction. The performance was verified by the detection of action potentials of primary neurons. The electrical noise of the custom-made MEA was equal to commercially available multielectrode arrays. Additionally, we demonstrated that structured coating with poly lysine, obtained with the aid of the same lithographic system, could be used to attach and guide neurons to designed structures. The process of neuron attachment and neurite guidance was investigated by light microscopy and charged particle microscopy....Continue Reading

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Citations

May 8, 2021·Colloids and Surfaces. B, Biointerfaces·L PasquardiniS Dirè

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Methods Mentioned

BETA
FCS
fluorescence imaging
x-ray
scanning electron microscopy
Fluorescence

Software Mentioned

POWD
AutoCAD
OriginPro

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