PMID: 489994Jan 1, 1979Paper

Technical improvements in the clotted plasma droplet MIF assay in vitro

Journal of Immunological Methods
G Sandru, P Veraguth

Abstract

The migration inhibitory activity of culture supernatants of rat and human lymphocytes stimulated with PHA and Con A-Sepharose was tested on cell migration from clotted plasma droplets. This technique was improved by using homologous as well as heterologous plasma and fibrinogen solutions for suspending the migratory cells, automatic micropipettes for performing the technique and purified cell populations as target. The effects of calcium chloride and of the cell concentration in the plasma droplets on the migration indices obtained in the MIF assay were tested.

References

Jan 1, 1978·Journal of Immunological Methods·G Sandru, L Sandru
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·R O MulhausenK Mellemgaard

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Citations

Dec 1, 1981·International Journal of Radiation Oncology, Biology, Physics·G Sandru, P Veraguth
Apr 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·A D AdoV I Dontsov

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