Technical note: developmental validation of a novel 6-dye typing system with 36 Y-STR loci.

International Journal of Legal Medicine
Weian DuLing Chen

Abstract

Y-chromosomal short tandem repeats (Y-STRs) have proven to be very useful in investigating sexual assault cases and in paternity lineage differentiation. However, currently available commercial Y-STR multiplex amplification systems bear the limitations in the identification of related males from the same paternal lineage due to there being an insufficient number of loci in any single amplification kit. The aim of this study was to establish and validate a novel 6-dye, 36-plex Y-STR multiplex amplification system that incorporated all of the loci present in the Yfiler™ Plus kit (DYS19, DYS385a/b, DYF387S1, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627, DYS635, Y_GATA_H4) as well as a further nine highly polymorphic Y-STR loci (DYS388, DYS444, DYS447, DYS522, DYS527a/b, DYS549, DYS596, DYS643). The novel system was optimized and validated by a series of studies that tested the effect of different PCR-based conditions as well as the species specificity, sensitivity, stability, stutter precision, suitability for use on DNA mixtures, reproducibility, and parallel testing of the system, as well as its performance on casework s...Continue Reading

References

Jan 1, 1997·International Journal of Legal Medicine·M A JoblingC Tyler-Smith
Jan 1, 1997·International Journal of Legal Medicine·M KayserL Roewer
Apr 28, 2004·Forensic Science International : Synergy·M HedmanA Sajantila
Jul 5, 2005·Forensic Science International : Synergy·R Y Y YongE P H Yap
Sep 26, 2006·International Journal of Legal Medicine·L GusmãoUNKNOWN International Society of Forensic Genetics
Nov 16, 2006·International Journal of Legal Medicine·Hwan Young LeeKyoung-Jin Shin
Mar 14, 2007·Forensic Science International : Synergy·Natsuko MizunoKentaro Kasai
May 21, 2009·Forensic Science, Medicine, and Pathology·Lutz Roewer
May 12, 2010·Forensic Science International. Genetics·M HedmanJ U Palo
May 27, 2011·Forensic Science International. Genetics·Kaye N BallantyneManfred Kayser
Jan 23, 2013·Forensic Science International. Genetics·Jonelle M ThompsonDouglas R Storts
Mar 12, 2013·Forensic Science International. Genetics·Soumaya Triki-FendriÁngel Carracedo
Apr 16, 2013·Forensic Science International. Genetics·Panayotis KatsaloulisPenelope Miniati
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 9, 2014·International Journal of Legal Medicine·Wenjuan ShanXiufen Zheng
Feb 18, 2014·Forensic Science International. Genetics·E Ramos-LuisA Carracedo
Aug 3, 2014·Forensic Science International. Genetics·Suhua ZhangChaoneng Ji
Sep 8, 2015·Forensic Science International. Genetics·Xueling OuHongyu Sun
Nov 4, 2015·Forensic Science International. Genetics·Liming LiShi Yan
Dec 17, 2015·International Journal of Legal Medicine·Ying WangHong-yu Sun
Jan 8, 2016·Forensic Science International. Genetics·Ines PickrahnFranz Neuhuber
Feb 5, 2016·Scientific Reports·Yingnan BianChengtao Li
Feb 10, 2016·Forensic Science International. Genetics·Jing XuShilin Li
Feb 21, 2016·Forensic Science International. Genetics·Rufeng BaiShuhua Ma
Feb 29, 2016·Forensic Science International. Genetics·Lei WangZhaoshu Zeng
Mar 5, 2016·International Journal of Legal Medicine·Rufeng BaiMs Shi
Nov 11, 2016·International Journal of Legal Medicine·Wenqiong ZhangDaixin Huang
Dec 3, 2016·International Journal of Legal Medicine·Weian DuChao Liu
Jan 10, 2017·Forensic Science International. Genetics·Giuseppe IacovacciFulvio Cruciani
Jan 17, 2017·International Journal of Legal Medicine·GuangLin HeYiPing Hou
Mar 21, 2017·Human Genetics·Manfred Kayser
Apr 1, 2017·Genetics and Molecular Research : GMR·H M GaoM X Zhang
Apr 14, 2017·International Journal of Legal Medicine·Shuanglin LiChao Liu
Nov 19, 2017·Forensic Science International. Genetics·Yuxiang ZhouJianhui Xie
Mar 9, 2018·Forensic Science International. Genetics·Shuqiang CaoWeibo Liang

❮ Previous
Next ❯

Citations

May 30, 2020·International Journal of Legal Medicine·Xingyi YangChao Liu
Jun 11, 2020·International Journal of Legal Medicine·Yuqing LiuWeibo Liang
May 7, 2020·International Journal of Legal Medicine·Jinding LiuGengqian Zhang
Apr 28, 2021·Scientific Reports·Julianna Kesselring RomeroEdna Sadayo Miazato Iwamura
Jun 11, 2021·Journal of Forensic Sciences·Lei ShangWanshui Li
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Jie Shang, Sheng-Ping Hu
© 2022 Meta ULC. All rights reserved