Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity 18 F-FDG injection

Medical Physics
Assen S KirovStephen B Solomon

Abstract

Genomic profiling of biopsied tissue is the basis for precision cancer therapy. However, biopsied materials may not contain sufficient amounts of tumor deoxyribonucleonic acid needed for the analysis. We propose a method to determine the adequacy of specimens for performing genomic profiling by quantifying their metabolic activity. We estimated the average density of tumor cells in biopsy specimens needed to successfully perform genomic analysis following the Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT) protocol from the minimum amount of deoxyribonucleonic acid needed and the volume of tissue typically used for analysis. The average 18 F-FDG uptake per cell was assessed by incubating HT-29 adenocarcinoma tumor cells in 18 F-FDG containing solution and then measuring their activity with a scintillation well counter. Consequently, we evaluated the response of two devices around the minimum expected activities which would indicate genomic profiling adequacy of biopsy specimens obtained under 18 F-FDG PET/CT guidance. Surrogate samples obtained using 18G core needle biopsies of gels containing either 18 F-FDG-loaded cells in the expected concentrations or the corresponding activi...Continue Reading

References

Nov 24, 2004·Physics in Medicine and Biology·S JanC Morel
Apr 2, 2005·Journal of Vascular and Interventional Radiology : JVIR·Stephen B Solomon
Mar 3, 2009·Radiology·Bingsheng HuangPek-Lan Khong
Jun 24, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Richard L WahlMartin A Lodge
Mar 4, 2011·Journal of Vascular and Interventional Radiology : JVIR·Servet TatliStuart G Silverman
Jun 7, 2011·The New England Journal of Medicine·Paul B ChapmanUNKNOWN BRIM-3 Study Group
Jun 28, 2011·AJR. American Journal of Roentgenology·Shuai LengCynthia H McCollough
Oct 27, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Juliano Julio CerciJoão Vicente Vitola
Dec 12, 2012·Cardiovascular and Interventional Radiology·E Ronan RyanStephen B Solomon
Apr 17, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Levi A Garraway
Sep 3, 2013·Techniques in Vascular and Interventional Radiology·Paul B Shyn
Sep 7, 2013·Medical Physics·James J HamillEmily B Martin
Dec 27, 2013·International Journal of Hepatology·John P McGahanMichael T Corwin
May 23, 2014·JAMA : the Journal of the American Medical Association·Mark G KrisPaul A Bunn
Feb 28, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Louise M FanchonAssen S Kirov
Apr 18, 2015·BMC Cancer·Nicola Normanno, Ian A Cree

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Citations

Jan 27, 2021·The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society of Radiopharmaceutica·Mateos BogoniAssen S Kirov
Apr 22, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Syamantak KhanGuillem Pratx
Mar 27, 2021·Physics in Medicine and Biology·David SarrutEmilie Roncali

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