Technical variations in prostatic immunohistochemistry: need for standardisation and stringent quality assurance in PSA and PSAP immunostaining

Journal of Clinical Pathology
M VarmaAnthony Rhodes

Abstract

To assess variations in prostate specific antigen (PSA) and prostate specific acid phosphatase (PSAP) immunohistochemistry with particular reference to the antibody type (monoclonal or polyclonal) and the tissues used for optimising immunostaining conditions and as external positive controls. A questionnaire was sent to all laboratories registered with the UK National External Quality Assurance Scheme for immunohistochemistry enquiring about the immunohistochemical methods routinely used for the diagnosis of prostate cancer. Responses were received from 220 (68%) laboratories. All UK respondents routinely performed PSA immunostaining but PSAP immunostaining was available in only 57% of these laboratories. Monoclonal anti-PSA, polyclonal anti-PSA, monoclonal anti-PSAP, and polyclonal anti-PSAP were used by 40%, 60%, 29%, and 27% of UK respondents, respectively. Benign prostate tissue was most commonly used to determine optimal antibody dilutions and as external quality control for PSA/PSAP, with only 6% and 3% of respondents, respectively, including high grade prostate cancer in the tissues used for these purposes. The wide variation in the methods used highlights the need for standardisation and more stringent quality assurance...Continue Reading

References

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Citations

May 26, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Neal S GoldsteinUNKNOWN Members of Ad-Hoc Committee On Immunohistochemistry Standardization
Mar 12, 2011·Indian Journal of Pathology & Microbiology·Nasir Ud DinSamina Mansoor
Jul 16, 2008·Annals of Diagnostic Pathology·Katia Ramos Moreira LeiteLuiz Heraldo Camara-Lopes
Sep 21, 2007·Histopathology·D M BerneyUNKNOWN Trans-Atlantic prostate group
Aug 8, 2009·International Journal of Cancer. Journal International Du Cancer·Anne J ZielinskiPierre-Yves Desprez
Jul 8, 2005·Expert Review of Proteomics·Till BraunschweigStephen M Hewitt
Sep 10, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Justin M M Cates, K Ashley Troutman
Mar 6, 2008·Archives of Pathology & Laboratory Medicine·Armita BahramiJae Y Ro

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