PMID: 591644Nov 1, 1977

Technique for microdissection and measurement in biopsies of human small intestine

Journal of Clinical Pathology
A FergusonF Allan

Abstract

A microdissection and measurement technique has been adapted for biopsy of human small intestine. Specimens fixed in alcohol and acetic acid are Schiff stained in bulk. Villi and crypts are then dissected out under a dissecting microscope, placed under a coverslip, examined, measured, and the number of mitoses in individual crypts counted. With this method specimens of normal small intestine have been found to have villi 500 microns to 1100 microns long and crypts 150 microns to 300 microns. These values were double those obtained when measuring sections of the same specimens stained with haematoxylin and eosin. The mean number of mitoses per crypt in normal duodenum and jejunum ranged from 1 to 12 and most of the cells in mitosis were in prophase or telophase. This rapid, sensitive, and inexpensive technique complements the available methods of measuring small intestinal architecture.

Citations

Dec 1, 1985·World Journal of Surgery·J B Bristol, R C Williamson
Sep 30, 1989·Lancet
Aug 1, 1996·The Australian and New Zealand Journal of Surgery·D R KingJ M King
Nov 1, 1988·Gut·G V AppletonR C Williamson
Mar 1, 1995·Digestive Diseases and Sciences·G D'ArgenioG Mazzacca
Oct 12, 2012·Digestive Diseases and Sciences·A G CumminsPhulwinder K Grover
Feb 1, 1988·Zentralblatt für Veterinärmedizin. Reihe A·A van Weeren-Keverling BuismanM A van der Velden
Nov 1, 1995·Veterinary Pathology·K C VarshneyG A Hall
Nov 2, 2018·Digestive Diseases and Sciences·Zenab Mustansir DudhwalaA G Cummins
Jul 1, 1990·The British Journal of Surgery·G V AppletonR C Williamson
Aug 1, 1989·The British Journal of Surgery·G V Appleton, R C Williamson
Apr 1, 1993·The British Journal of Surgery·M G ThomasR C Williamson
Jul 31, 2008·Journal of Pediatric Gastroenterology and Nutrition·A G CumminsFiona M Thompson
Nov 1, 1995·Scandinavian Journal of Gastroenterology·C CiacciG Mazzacca
Jun 27, 2020·BMJ Open Gastroenterology·Zenab Mustansir DudhwalaAdrian Gerard Cummins

Related Concepts

Biopsy
Cell Density
Tissue Dissection
Intestines, Small
Micromanipulation
M Phase, Mitotic

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