Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)

Biomedical Optics Express
Dinglong MaLaura Marcu

Abstract

We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).

References

Jan 26, 2012·Annals of Biomedical Engineering·Laura Marcu
Sep 3, 2013·Photodiagnosis and Photodynamic Therapy·Charlotte KallawayNick Stone
Mar 13, 2014·Lasers in Surgery and Medicine·Anna C CroceMariapia Vairetti
Apr 3, 2014·The Review of Scientific Instruments·Diego R YankelevichLaura Marcu

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Citations

Feb 19, 2016·IEEE Transactions on Medical Imaging·Dimitris GorpasLaura Marcu
Jun 24, 2017·Methods and Applications in Fluorescence·Farzad FereidouniLaura Marcu
Nov 4, 2017·Physics in Medicine and Biology·Jennifer E PhippsLaura Marcu
Feb 13, 2018·Clinical and Experimental Dermatology·D GorpasN Fazel
Dec 31, 2017·Journal of Biomedical Optics·Stephen A BoppartHenricus J C M Sterenborg
Dec 24, 2018·Journal of Biomedical Optics·Xiangnan ZhouLaura Marcu
May 2, 2019·Optics Letters·Benjamin E SherlockLaura Marcu
Apr 21, 2021·The Journal of Surgical Research·Mark MarsdenMichael J Campbell

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