Technique for retransplanting heterotopic heart grafts in mice

Microsurgery
Qi LiFadi G Lakkis

Abstract

Removal of a transplanted organ from its original recipient and retransplanting it into a new host is an important method to study the role of the graft in the rejection process. Here we describe a novel technique of heart retransplantation in the mouse. In this technique, a primarily vascularized heart graft is anastomosed to the abdominal aorta and inferior vena cava of a syngeneic or immunodeficient allogeneic mouse, using standard techniques. Either 10 or 70 days later, the same graft is retransplanted into the abdomen of a second mouse by end-to-side anastomosis of the donor (first recipient) aortic and inferior vena cava's cuffs to the second recipient's abdominal aorta and inferior vena cava, respectively. A greater than 90% success rate was achieved by using this microsurgical technique. This method should be useful for studying intragraft factors, such as ischemia-reperfusion injury and donor antigen-presenting cells, on the outcomes of transplantations.

References

Oct 12, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·M Niimi

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Citations

Jul 5, 2011·Asian Journal of Surgery·Song SuOliver Witzke
Aug 7, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T NozakiR L Fairchild

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