PMID: 2482708Jan 1, 1989Paper

Techniques for sperm immobilization test

Archives of Andrology
S Isojima, K Koyama


Several semiquantitative, quantitative, and microassay techniques had been developed to detect antibodies bound to human spermatozoa: sperm agglutination test (SAT), sperm immobilization test (SIT), immunofluorescence test, radioantiglobulin test, enzyme-linked immunosorbent assay (ELISA), mixed erythrocyte-spermatozoa antiglobulin reaction (MAR), "Panning" test, and immunobead test (IBT). Clinical application of these techniques include (a) detection of sperm immobilizing antibodies in sera of sterile women, (b) follow-up study of sperm immobilizing antibodies, and (c) detection of sperm immobilizing antibodies in cervical mucus and other secretions. The chemical structure of antigen epitope corresponding to Mab H6-3C4 may recognize the internally located repetitive unbranched N-acetyllactosamine structure, regardless of terminal substitution at Gal (i.e., sialyl-i as well as i structure). The majority of sperm-immobilization (SI) positive women's sera were absorbed with carbohydrate components on ejaculated sperm, but only one serum competed with Mab H6-3C4 on binding to sperm except a serum from whom lymphocytes were donated to make Mab H6-3C4. The SI agglutinating antibodies (Abs) in women's sera were raised to the carbohyd...Continue Reading


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