PMID: 6168034May 1, 1981Paper

Techniques for studying adipocytes

Stain Technology
G J Hausman

Abstract

Various fixatives as well as tissue and slide handling procedures have been evaluated in attempts to demonstrate adipocytes histochemically while maintaining cell and tissue integrity. The optimal procedure for analysis of immature adipose depots consists of the following steps: 1) fresh, unfixed tissues are rapidly in isopentane quenched in a liquid nitrogen bath; 2) cryostat sections are cut, removed from the knife with a room temperature slide, and then air dried for 5-10 minutes; 3) slides can be stained directly with picro-Ponceau or toluidine blue procedures or with oil red O following fixation for 30 minutes in cold (4 C) 10% formalin-CaCl2 (1.25%). For analysis of mature rat adipose depots steps 2 and 3 are modified as follows: 2) cryostat sections are removed from the knife with a cold slide (-20 C) and dried for 30 minutes at 4 C; 3) the mounted sections are stained with oil red O following fixation for 30 minutes in cold (4 C) 10% formalin-HgCl2 (2.5%). When procedures described above for immature adipose depots are combined with esterase staining, adipocyte cytoplasm is clearly demonstrated. These procedures allow the routine use of fresh frozen, unfixed cryostat sections in studies of adipose cellularity.

Citations

Jan 1, 1988·Cell and Tissue Research·T G RamsayR J Martin
Nov 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H R GaskinsG J Hausman
Nov 11, 2006·Diabetologia·Y TchoukalovaM Jensen
Oct 8, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T G Ramsay, R W Rosebrough
Mar 1, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Haiyen E ZhauRuoxiang Wang
Jan 23, 2014·Molecular and Cellular Endocrinology·Carlos F LagosCarlos E Fardella
Jun 16, 2007·In Vitro Cellular & Developmental Biology. Animal·Chang-qing QuGong-she Yang
May 1, 1995·In Vitro Cellular & Developmental Biology. Animal·J T Wright, G J Hausman
Jan 5, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Shinya KatoNobuhiko Miwa
Aug 17, 2010·Biochemical and Biophysical Research Communications·Masamitsu KonnoMakoto Asashima
Mar 14, 2009·Journal of Tissue Engineering and Regenerative Medicine·Aditee Kurane, Naren Vyavahare
Mar 19, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Perumal NagarajanSubeer S Majumdar
Mar 5, 2013·Development, Growth & Differentiation·Masamitsu KonnoHideshi Ishii
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Oct 9, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·E S Baskin-BeyG J Gores

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