Techniques for the in vivo assessment of cardio-renal function in zebrafish (Danio rerio) larvae.

The Journal of Physiology
Sebastien A RiderJohn J Mullins

Abstract

Zebrafish, a well-established vertebrate model, offer unique advantages for assessing renal function and physiology. Assays determining renal glomerular function based on cardiovascular erythrocyte flow and reduction of injected FITC-inulin were developed, each validated using the nephrotoxin gentamicin. Bland–Atlman analysis showed a strong association between measurements of the rate of inulin excretion and that of fluorescent reduction from the arterial vasculature. Reduced renal clearance of inulin, resulting from gentamicin or NaCl loading, was concurrent with reduced erythrocyte velocity, and yolk sac and pericardium oedema. These techniques, assessing pronephric function, highlight the potential for in vivo physiological study in this genetically tractable model.

References

Jul 3, 2003·Trends in Cell Biology·Iain Drummond
Jul 19, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Volker Vallon
Jan 14, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adrian J HillWarren Heideman
Dec 31, 2004·American Journal of Physiology. Renal Physiology·Dirk M HentschelJoseph V Bonventre
Jul 28, 2006·Methods : a Companion to Methods in Enzymology·Nicole A SabaliauskasKeith C Cheng
May 31, 2007·The Journal of Endocrinology·Kazuyuki Hoshijima, Shigehisa Hirose
Aug 19, 2007·American Journal of Physiology. Renal Physiology·Dirk M HentschelMario Schiffer

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Citations

Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Gary F Gerlach, Rebecca A Wingert
Jun 13, 2014·Genesis : the Journal of Genetics and Development·Paul T Kroeger, Rebecca A Wingert
Jun 20, 2015·Stem Cells International·Kristen K McCampbellRebecca A Wingert
May 15, 2015·Current Pathobiology Reports·Robert A McKee, Rebecca A Wingert
Feb 16, 2017·Scientific Reports·Mohamed A ElmonemElena Levtchenko
Mar 17, 2016·World Journal of Stem Cells·Bridgette E Drummond, Rebecca A Wingert
Jun 28, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Matthew R StoyekFrank M Smith
Jul 24, 2015·American Journal of Physiology. Renal Physiology·Sebastien A RiderJohn J Mullins
Jul 14, 2018·Frontiers in Pediatrics·Jochen GehrigJens H Westhoff
Jul 13, 2012·The Journal of Physiology·Pontus B Persson
Feb 10, 2017·American Journal of Physiology. Renal Physiology·Sebastien A RiderJohn J Mullins
Sep 12, 2018·Cells·Mohamed A ElmonemElena N Levtchenko
Nov 3, 2021·Nature Communications·Gianfranco MatroneJohn P Cooke

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