Techniques for the induction of human pluripotent stem cell differentiation towards cardiomyocytes

Journal of Tissue Engineering and Regenerative Medicine
Jarosław LewandowskiMaciej Kurpisz

Abstract

The derivation of pluripotent stem cells from human embryos and the generation of induced pluripotent stem cells (iPSCs) from somatic cells opened a new chapter in studies on the regeneration of the post-infarction heart and regenerative medicine as a whole. Thus, protocols for obtaining iPSCs were enthusiastically adopted and widely used for further experiments on cardiac differentiation. iPSC-mediated cardiomyocytes (iPSC-CMs) under in vitro culture conditions are generated by simulating natural cardiomyogenesis and involve the wingless-type mouse mammary tumour virus integration site family (WNT), transforming growth factor beta (TGF-β) and fibroblast growth factor (FGF) signalling pathways. New strategies have been proposed to take advantage of small chemical molecules, organic compounds and even electric or mechanical stimulation. There are three main approaches to support cardiac commitment in vitro: embryoid bodis (EBs), monolayer in vitro cultures and inductive co-cultures with visceral endoderm-like (END2) cells. In EB technique initial uniform size of pluripotent stem cell (PSC) colonies has a pivotal significance. Hence, some methods were designed to support cells aggregation. Another well-suited procedure is based o...Continue Reading

References

Dec 31, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·F B SmithG D Lowe
Feb 13, 2001·Genes & Development·E Tzahor, A B Lassar
Feb 9, 2002·Circulation Research·W-H ZimmermannT Eschenhagen
Sep 21, 2002·Circulation Research·Chunhui XuMelissa K Carpenter
Apr 2, 2003·Circulation·Tomosaburo TakahashiRichard T Lee
Mar 5, 2004·Journal of Cell Science·Martin F PeraChristine Mummery
Sep 28, 2004·Nature Biotechnology·Izhak KehatLior Gepstein
Oct 7, 2004·Developmental Biology·William T PuSeigo Izumo
Nov 1, 2005·Nature Biotechnology·Kevin A D'AmourEmmanuel E Baetge
Aug 4, 2006·Biomaterials·Jeffrey C MohrSean P Palecek
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Sep 14, 2006·Tissue Engineering·Milica RadisicGordana Vunjak-Novakovic
Sep 21, 2006·Chemistry & Biology·Ai-Sun TsengMark T Keating
Jan 16, 2007·Circulation Research·Oren CaspiShulamit Levenberg
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irit HuberLior Gepstein
Apr 26, 2007·Annual Review of Cell and Developmental Biology·Radwan Abu-Issa, Margaret L Kirby
May 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shuichi UenoCharles E Murry
Oct 20, 2007·The EMBO Journal·Raheem PeeraniPeter W Zandstra
Oct 24, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Stefanie DimmelerAndreas M Zeiher
Nov 6, 2007·Journal of the American College of Cardiology·Oren CaspiLior Gepstein
Nov 21, 2007·Differentiation; Research in Biological Diversity·Ralph GraichenBruce P Davidson
Feb 19, 2008·Nature Biotechnology·Kenji OsafuneDouglas A Melton
Apr 26, 2008·Circulation Research·Linda W van LaakeChristine L Mummery

❮ Previous
Next ❯

Citations

Aug 5, 2016·BMC Systems Biology·Losiana NayakRajat K De
Jul 19, 2017·ACS Applied Materials & Interfaces·Jiaxian WangMinglong Chen
Mar 15, 2018·Stem Cells International·Heiko LemckeRobert David
Sep 11, 2020·Oxidative Medicine and Cellular Longevity·Jialiang LiangPing Zhu
Jun 22, 2017·Biomedical Microdevices·Samad AhadianAli Khademhosseini
Mar 8, 2017·Journal of Cardiovascular Translational Research·Teresa Simon-YarzaDidier Letourneur
Sep 6, 2020·International Journal of Molecular Sciences·Melania LippiElena Sommariva
Oct 10, 2020·International Journal of Immunopathology and Pharmacology·Jun WanShaodong Qiu
Oct 25, 2020·Stem Cell Reviews and Reports·Pallavi PushpMukesh Kumar Gupta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.