PMID: 11343926May 10, 2001Paper

Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA

Trends in Biochemical Sciences
J P MuyrersA F Stewart

Abstract

Driven by the needs of functional genomics, DNA engineering by homologous recombination in Escherichia coli has emerged as a major addition to existing technologies. Two alternative approaches, RecA-dependent engineering and ET recombination, allow a wide variety of DNA modifications, including some which are virtually impossible by conventional methods. These approaches do not rely on the presence of suitable restriction sites and can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule. Furthermore, ET recombination can be used for direct subcloning and cloning of DNA sequences from complex mixtures, including bacterial artificial chromosomes and genomic DNA preparations. The strategies reviewed in this article are applicable to modification of DNA molecules of any size, including very large ones, and present powerful new avenues for DNA manipulation in general.

References

Jan 1, 1992·Trends in Genetics : TIG·N L Sternberg
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Jun 16, 1989·Science·M O'ConnorW Bender
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T L Orr-WeaverR J Rothstein
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A PoustkaH Lehrach
Sep 1, 1994·Microbiological Reviews·S C KowalczykowskiW M Rehrauer
Jan 1, 1994·Molecular Microbiology·R KolodnerC Luisi-DeLuca
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·M MesserleU H Koszinowski
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R JessenS Lin
Oct 15, 1998·Nature Genetics·Y ZhangA F Stewart
Feb 26, 1999·Nucleic Acids Research·J P MuyrersA F Stewart
Aug 14, 1999·Nucleic Acids Research·P O AngrandA F Stewart
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Kuzminov
Jan 5, 2000·Journal of Human Hypertension·C M PayneJ J Mullins
Mar 10, 2000·Genomics·F HillW Ansorge
Apr 18, 2000·Gene·K C MurphyA R Poteete
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
May 29, 2000·Trends in Genetics : TIG·W BruneU H Koszinowski
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jan 3, 2001·Genesis : the Journal of Genetics and Development·S SwaminathanS K Sharan
Mar 21, 2001·EMBO Reports·J P MuyrersA F Stewart

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Citations

Aug 31, 2006·Plant Molecular Biology·Qi-Jun ChenXue-Chen Wang
Jan 23, 2009·Molecular Biotechnology·Shuwen WangJiyue Zhu
Mar 23, 2005·Trends in Biotechnology·Quinn Lu
Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter
Oct 15, 2003·Gene·Donald L CourtShyam K Sharan
Apr 9, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pier Paolo PeruzziRichard Wade-Martins
Sep 23, 2006·Nature Methods·Mihail SarovA Francis Stewart
Jul 6, 2006·The Journal of Biological Chemistry·Zengru WuCharles E Bell
Apr 12, 2002·Cloning and Stem Cells·H Cooke
May 15, 2002·Cloning and Stem Cells·Lluís Montoliu
Feb 24, 2004·Nucleic Acids Research·Jeffrey S SwersK Dane Wittrup
May 1, 2008·Development, Growth & Differentiation·Shin-ichi Higashijima
Jun 12, 2013·Applied and Environmental Microbiology·Brian H KvitkoHerbert P Schweizer
Jun 25, 2009·Molekuliarnaia biologiia·A Iu GulevichS V Mashko
Aug 20, 2009·Annual Review of Genetics·Steve D M BrownJohn M Hancock
Sep 24, 2010·Journal of Biomedicine & Biotechnology·Sara Pérez-Luz, Javier Díaz-Nido
Dec 3, 2005·The Journal of Clinical Investigation·Jeffrey M ZigmanJoel K Elmquist
Jul 31, 2010·BMC Molecular Biology·Marcello MarescaA Francis Stewart
Jan 8, 2004·BMC Biotechnology·George Kotzamanis, Clare Huxley
Mar 20, 2008·Genome Biology·Jonathan E AllenTom R Slezak
Aug 23, 2012·PLoS Pathogens·April J SchumacherSandra K Weller
Dec 21, 2006·Genetics·Hajime TakeuchiDieter Egli
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Stefan PorubskyHermann-Josef Gröne
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Li LinBaojian Li
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ralf KittlerFrank Buchholz
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jinjin ZhangCharles E Bell
Dec 27, 2013·Nucleic Acids Research·Hailong WangA Francis Stewart

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