Abstract
In response to cellular stress or damage, proliferating cells can induce a specific program that initiates a state of long-term cell-cycle arrest, termed cellular senescence. Accumulation of senescent cells occurs with organismal aging and through continual culturing in vitro. Senescent cells influence many biological processes, including embryonic development, tissue repair and regeneration, tumor suppression, and aging. Hallmarks of senescent cells include, but are not limited to, increased senescence-associated β-galactosidase activity (SA-β-gal); p16INK4A, p53, and p21 levels; higher levels of DNA damage, including γ-H2AX; the formation of Senescence-associated Heterochromatin Foci (SAHF); and the acquisition of a Senescence-associated Secretory Phenotype (SASP), a phenomenon characterized by the secretion of a number of pro-inflammatory cytokines and signaling molecules. Here, we describe protocols for both replicative and DNA damage-induced senescence in cultured cells. In addition, we highlight techniques to monitor the senescent phenotype using several senescence-associated markers, including SA-β-gal, γ-H2AX and SAHF staining, and to quantify protein and mRNA levels of cell cycle regulators and SASP factors. These meth...Continue Reading
Citations
Mar 7, 2019·The Journal of Pharmacology and Experimental Therapeutics·Fa-Ju ChenJing-Shan Shi
Dec 14, 2019·Aging Cell·Luigi FerrucciRafael de Cabo
Mar 11, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Giulia JannoneEtienne M Sokal
Jul 20, 2019·European Journal of Oral Sciences·Lisa PrintzellJan E Ellingsen
May 20, 2020·International Journal of Molecular Sciences·Jaqueline HerrmannMirjam Schuchardt
Aug 4, 2020·Veterinary Pathology·Jessica BeckCurtis Harris
May 11, 2020·Aging·Yuan ZhangYunhua Chang
Jul 28, 2020·Aging·Fei XuHaitao Wang
Aug 30, 2018·Scientific Reports·Barbara Kuźnar-KamińskaHalina Batura-Gabryel
Jun 7, 2019·Cell & Bioscience·Bin LinJing Xu
Sep 27, 2020·Cells·Gisela Machado-OliveiraOtília V Vieira
Aug 6, 2019·Biochemia Medica·Slavica DodigIvan Pavić
Nov 20, 2019·Molecular Biology Reports·Ilknur Sur-ErdemAngelika A Noegel
Mar 28, 2020·Oxidative Medicine and Cellular Longevity·Parameswaran G SreekumarRam Kannan
May 1, 2020·Cancers·Lía AlzaJudit Herreros
Sep 28, 2018·Stem Cell Research & Therapy·Giuliana Di RoccoGabriele Toietta
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Xueke ZhouXin Li
Dec 8, 2019·Nature Communications·Clément CrochemoreMiria Ricchetti
Sep 14, 2018·Frontiers in Pharmacology·Ji-Ye KeeSeung-Heon Hong
Mar 11, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Martyn T SmithMark Fielden
Nov 22, 2020·Cancer Research·Heather FairfieldMichaela R Reagan
Feb 10, 2021·Cancers·Philipp Ernst, Florian H Heidel
Oct 27, 2020·Stem Cells International·Provvidenza M AbruzzoCarlo Ventura
Apr 4, 2021·Cancers·Lieze BerbenSigrid Hatse
Feb 19, 2021·Aging·Rachael E SchwartzGerald S Shadel
May 1, 2021·Cells·Karen E HemmingsKaren E Porter
May 28, 2021·Histochemistry and Cell Biology·Ellaine SalvadorCarola Y Förster
Jul 3, 2021·Cancers·Nadine WiesmannJuergen Brieger
Apr 27, 2021·Aging·Alfredo Martínez-GutiérrezAlejandro Vaquero
Aug 8, 2021·Cells·Martina Rossi, Kotb Abdelmohsen
Aug 10, 2021·Frontiers in Oncology·Aling ShenFenglin Chen
Aug 21, 2021·Oxidative Medicine and Cellular Longevity·Wenwen LiuZelan Hu
Oct 7, 2020·Nature Communications·Amr OmerImed-Eddine Gallouzi
Dec 4, 2021·Frontiers in Public Health·Chuan SunGenxiang Mao