Technologies and methods for sample pretreatment in efficient proteome and peptidome analysis

Proteomics
Xiaogang JiangHanfa Zou

Abstract

Although great progresses have been made in proteomics during the last decade, proteomics is still in its infancy. Extreme complexity of proteome sample and large dynamic range of protein abundance overwhelm the capability of all currently available analytical platforms. Sample pretreatment is a good approach to reduce the complexity of proteome sample and decrease the dynamic range. In this article, we present an overview of different technologies and methods for sample pretreatment in efficient proteome and peptidome analysis. Methods for isolation of rare amino acid-containing peptides, terminal peptides, PTM peptides and endogenous peptides are reviewed. In addition, two automated sample pretreatment technologies, i.e. automated sample injection and on-line digestion, are also covered.

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Citations

Aug 19, 2011·Se pu = Chinese journal of chromatography·Hongwei LiuMingliang Ye
Dec 24, 2009·Journal of Biomedicine & Biotechnology·Muriel De BockMarianne Fillet
Aug 18, 2009·Expert Review of Proteomics·Lianghai HuHanfa Zou
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May 17, 2015·Journal of Proteomics·S MahboobM S Baker
May 4, 2011·Proteomics·Simon FaulknerDermot Morris
Dec 29, 2011·Journal of Biomedicine & Biotechnology·Inez FinoulstPeter Verhaert
Jun 20, 2013·Journal of Pharmaceutical and Biomedical Analysis·Youji ShimazakiMasaki Saito
Jul 1, 2010·Journal of Biomedicine & Biotechnology·Raquel González-FernándezJesús V Jorrín-Novo

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