TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes.

Nature Communications
Lisa WetzelThomas Wollert

Abstract

The accumulation of protein aggregates is involved in the onset of many neurodegenerative diseases. Aggrephagy is a selective type of autophagy that counteracts neurodegeneration by degrading such aggregates. In this study, we found that LC3C cooperates with lysosomal TECPR1 to promote the degradation of disease-related protein aggregates in neural stem cells. The N-terminal WD-repeat domain of TECPR1 selectively binds LC3C which decorates matured autophagosomes. The interaction of LC3C and TECPR1 promotes the recruitment of autophagosomes to lysosomes for degradation. Augmented expression of TECPR1 in neural stem cells reduces the number of protein aggregates by promoting their autophagic clearance, whereas knockdown of LC3C inhibits aggrephagy. The PH domain of TECPR1 selectively interacts with PtdIns(4)P to target TECPR1 to PtdIns(4)P containing lysosomes. Exchanging the PH against a tandem-FYVE domain targets TECPR1 ectopically to endosomes. This leads to an accumulation of LC3C autophagosomes at endosomes and prevents their delivery to lysosomes.

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Citations

Nov 6, 2020·Protein & Cell·Linsen LiQing Zhong
Nov 21, 2020·Autophagy·Milana FraibergZvulun Elazar
Mar 16, 2021·Frontiers in Oncology·Maria Paz Hernández-CáceresCristina Bertocchi
May 2, 2021·The EMBO Journal·Marianna CarinciFrancesco Cecconi
Jul 25, 2021·Nature Reviews. Molecular Cell Biology·Yan G ZhaoHong Zhang
Aug 8, 2021·International Journal of Molecular Sciences·SeJeong KimKyoungJoo Cho
Sep 18, 2021·Frontiers in Immunology·Andreas RonitTrine H Mogensen

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Datasets Mentioned

BETA
GTX130212

Methods Mentioned

BETA
co-immunoprecipitation
co-IP
co-IPs
light electron microscopy
PCR
gene knockout
size-exclusion chromatography
lipidation
Transfection

Software Mentioned

IMOD
Coloc2
ec
Fiji
Leica
OriginPro
CLEM
Zeiss ZEN

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