Tegillarca granosa extract Haishengsu inhibits the expression of P-glycoprotein and induces apoptosis in drug-resistant K562/ADM cells

Pharmaceutical Biology
Guang-Yao LiLe-Xin Wang

Abstract

This study was designed to investigate the effect and molecular mechanisms of Haishengsu (HSS), a protein extract from a shellfish Tegillarca granosaL., on a drug resistant leukemia cell line. Cultured K562/Adriamycin (ADM) cells were treated with HSS at 10, 20 and 40 microg/mL, respectively. The apoptosis and expression of p-glycoprotein was evaluated by flow cytometry. Expressions of caspase-3 and Bcl-2 were also evaluated. There was a significant dose-dependent increase in the apoptosis in the HSS treated K562/ADM cells (P < 0.05 and 0.01, respectively). The p-glycoprotein expression in the 40 microg/mL HSS group (14.8%) was lower than in the control (16.9%, P < 0.05) and the 10 microg/mL HSS group (7.3%, P < 0.05), but it was similar to the HSS 20 microg/mL group (10.7%, P > 0.05). The expressions of apoptosis-stimulating protein caspase-3 protein were increased, whereas the expressions of apoptosis-suppressing Bcl-2 were decreased in the HSS groups, as compared with the levels in the control group (P < 0.05). We conclude that HSS induces apoptosis of the Adriamycin-resistant K562/ADM cells. The enhanced expressions in caspase-3 and the reduced expressions in Bcl-2 protein may have contributed to the apoptosis-stimulating e...Continue Reading

References

Oct 15, 1976·International Journal of Cancer. Journal International Du Cancer·E KleinF Vánky
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Aug 15, 1997·The Biochemical Journal·G M Cohen
Dec 4, 2003·Current Opinion in Cell Biology·Kelly M Boatright, Guy S Salvesen
Dec 21, 2006·Annals of Internal Medicine·Hagop M KantarjianJorge Cortes
Oct 12, 2007·Journal of Biochemistry and Molecular Biology·Won-Kyo JungSe-Kwon Kim
Aug 7, 2008·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·Guang-Yao LiLe-Xin Wang

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Citations

Feb 25, 2014·Apoptosis : an International Journal on Programmed Cell Death·Jian-Guo SunFei-Yan Liu
Apr 6, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuping JiaShuntai Chen
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guang-Yao LiMing Hou
Jul 3, 2021·Biomedicines·Assunta SaideChiara Lauritano

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