Telmisartan attenuates kidney apoptosis and autophagy-related protein expression levels in an intermittent hypoxia mouse model

Sleep & Breathing = Schlaf & Atmung
Xiao-Bin ZhangHenry Chidozie Ewurum

Abstract

Obstructive sleep apnea (OSA) is associated with renal impairs. As a novel pathophysiological hallmark of OSA, chronic intermittent hypoxia (CIH) enhances apoptosis and autophagy. The present study aims to evaluate the effect of telmisartan on CIH-induced kidney apoptosis and autophagy in a mouse model of OSA. Mice were randomly allocated to normoxia, CIH, and CIH+telmisartan groups (n = 12 in each group). The CIH exposure duration was 12 weeks. Mice in the CIH+telmisartan group received telmisartan administration. The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and western blotting of Bax and cleaved caspase-3 were conducted for evaluating apoptosis in kidney tissue. While the autophagy-related proteins, beclin-1 and LC3, were also observed via western blotting. The percentage of apoptotic cell in the CIH group was significantly higher than that of normoxia group; meanwhile, Bax and cleaved caspase-3 protein levels were increased in the CIH group than those of normoxia group (all p < 0.05). Compared with the normoxia group, mice in the CIH group had greater autophagy-related proteins (beclin-1 and LC3) expression. When compared to the CIH group, both the renal apoptosis and autophagy in the CIH+t...Continue Reading

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Citations

Mar 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng GuanEn-Sheng Ji
Jan 7, 2021·International Journal of Molecular Sciences·Christopher L MooreAlexei G Basnakian
May 5, 2021·Asia-Pacific Journal of Clinical Oncology·Yan WangLixiang Xue

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Methods Mentioned

BETA
protein assay
enzyme-linked immunosorbent assay
transmission electron microscopy

Software Mentioned

ImageJ
GraphPad Prism

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