PMID: 11323394Apr 27, 2001Paper

Telomerase activation and p53 mutations in urethane-induced A/J mouse lung tumor development

Carcinogenesis
J OhnoK Shimokata

Abstract

The mouse telomerase holoenzyme, which synthesizes telomeric DNA de novo, is a ribonucleoprotein complex that includes the mouse telomerase RNA component (mTERC), mouse telomerase-associated protein (mTEP1) and mouse telomerase reverse transcriptase (mTERT). To determine the role of telomerase in urethane-induced lung tumorigenesis in A/J mice we examined telomerase activity and the expression of each telomerase subunit in 20 tumor samples, harvested at 16, 28, 40 and 50 weeks after urethane treatment. The telomeric repeat amplification protocol assay showed that statistically significant telomerase activation occurred both early and late in tumorigenesis. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed that mRNA expression levels of mTEP1 and mTERT were up-regulated during tumor progression, while mTERC expression was not significantly different between tumors and normal lung. We further examined mTEP1 protein expression in normal lung tissue and lung tumors; western blot analysis showed preferential expression of mTEP1 protein in lung tumors compared with normal lung and immunohistochemistry revealed that a majority of the adenoma cells were positively stained in the nucleus, whereas only a...Continue Reading

Citations

Feb 28, 2007·Toxicologic Pathology·Nobuko WakamatsuRobert C Sills
Sep 2, 2005·Molecular and Cellular Biology·Masahiro TakakuraJerry W Shay
Jan 20, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G AltavillaA Corallini
Jan 26, 2010·International Journal of Experimental Pathology·Jirí SvecJirí Pácha
May 4, 2012·Journal of Tissue Engineering and Regenerative Medicine·Wan Safwani Wan Kamarul ZamanKien Hui Chua
Jun 13, 2015·BioMed Research International·Amelia KellarDon Morris
Aug 4, 2001·The American Journal of Pathology·K S Elenitoba-Johnson
Apr 28, 2012·Biochemical and Biophysical Research Communications·Manali AggrawalLiang Xue
Sep 28, 2017·Cancer Biomarkers : Section a of Disease Markers·Soo-Jin KimKyung-Taek Rim
Sep 13, 2003·World Journal of Gastroenterology : WJG·Yun-Feng PiaoPu-Jun Gao

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