Telomerase reverse transcriptase methylation predicts lymph node metastasis and prognosis in patients with gastric cancer

OncoTargets and Therapy
Yongxin WuZhen Zhang

Abstract

Telomerase activity is associated with cellular immortalization and is present in most human tumors but absent in normal tissues. The activity of telomerase reverse transcriptase (TERT), a catalytic telomerase subunit, correlates with telomerase activity in tumors. The objective of this study was to investigate TERT promoter methylation and its prognostic impact in gastric cancer (GC). The analysis of TERT promoter methylation was performed in tumors and corresponding normal tissues of 116 patients with GC using a highly sensitive Sequenom Epityper assay. The expression of TERT in GC tissues was measured by quantitative real-time polymerase chain reaction. The levels of TERT promoter methylation in GC samples were significantly higher than in normal adjacent tissues (P=0.002). Hypermethylation of TERT promoter was associated with high T-stage (P=0.024), late N-stage (P=0.006), and lymphovascular/neural invasion (P=0.035), without correlation with age, sex, or histological grade. Simple linear regression analysis showed that TERT mRNA correlated positively with TERT methylation (R (2)=0.562, P=0.001). Also, higher TERT mRNA expression was related to hypermethylation of TERT promoter in GC samples (P=0.005). Univariate analysis d...Continue Reading

Citations

Mar 13, 2017·The Journal of Molecular Diagnostics : JMD·Seungjae LeeArmita Bahrami
Oct 26, 2018·The Journal of Clinical Investigation·Donghyun D LeeUri Tabori
Aug 3, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kazuya HaraguchiKazuhiro Tominaga
Jul 4, 2020·Journal of Gastrointestinal Cancer·Sogand VahidiAli Akbar Samadani
Apr 4, 2021·Cancers·Fatma Dogan, Nicholas R Forsyth
Nov 2, 2021·The Journal of Clinical Investigation·Donghyun D LeeUri Tabori

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Methods Mentioned

BETA
surgical resection
PCR

Software Mentioned

Epityper
Sequenom
Sequenom Epityper
MassARRAY
SPSS17

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