Telomere and telomerase in the initial stage of immortalization of esophageal epithelial cell

World Journal of Gastroenterology : WJG
Zhong-Ying ShenYi Zeng

Abstract

To search for the biomarker of cellular immortalization, the telomere length, telomerase activity and its subunits in cultured epithelial cells of human fetal esophagus in the process of immortalization. The transgenic cell line of human fetal esophageal epithelium (SHEE) was established with E(6)E(7) genes of human papillomavirus (HPV) type 18 in our laboratory. Morphological phenotype of cultured SHEE cells from the 6th to 30th passages, was examined by phase contrast microscopy, the telomere length was assayed by Southern blot method, and the activity of telomerase was analyzed by telomeric repeat amplification protocol (TRAP). Expressions of subunits of telomerase, hTR and hTERT, were assessed by RT-PCR. DNA content in cell cycle was detected by flow cytometry. The cell apoptosis was examined by electron microscopy (EM) and TUNEL label. SHEE cells from the 6th to 10th passages showed cellular proliferation with a good differentiation. From the 12th to the 16th passages, many senescent and apoptotic cells appeared, and the telomere length sharply shortened from 23kb to 17kb without expression of hTERT and telomerase activity. At the 20th passage, SHEE cells overcame the senescence and apoptosis and restored their proliferati...Continue Reading

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Citations

Nov 20, 2002·World Journal of Gastroenterology : WJG·Zhong-Ying ShenYi Zeng
Sep 13, 2003·World Journal of Gastroenterology : WJG·Qing-You DuSheng-Qi Wang
Mar 25, 2004·World Journal of Gastroenterology : WJG·Xi-Mei ChenXia Zhang
Jan 1, 2003·World Journal of Gastroenterology : WJG·Zhi-Ming HaoGuang-Xiao Yang
Aug 14, 2002·World Journal of Gastroenterology : WJG·Xi-Xian YaoZhong-Cheng Sun
Nov 8, 2003·World Journal of Gastroenterology : WJG·Lian-Fu ZuoJiang-Hui Liu
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xiao-Yuan XieZhong-Ying Shen
Jun 12, 2003·World Journal of Gastroenterology : WJG·Zhong-Ying ShenYi Zeng
Jun 5, 2002·World Journal of Gastroenterology : WJG·Jun HouDeng-Gui Wen

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