Sep 14, 2013

Telomere length and gene expression of shelterin in CD3(+) T cell of severe aplastic anemia patients

Zhonghua yi xue za zhi
Ting WangZong-hong Shao

Abstract

To explore the changes in telomere length and gene expression of complex shelterin (composed of 6 core components: TRF1, TRF2, POT1, TIN2, TPP1 and RAP1) in severe aplastic anemia (SAA). Bone marrow samples were obtained from 20 SAA patients and 10 normal controls. CD3(+)T cells were sorted by immunomagnetic separation. Telomere length was tested by Southern blot and the gene expressions of TRF1, TRF2, POT1, TIN2, TPP1 and RAP1 were detected by reverse transcription-PCR(RT-PCR). Telomeres of CD3(+)T cells were found significantly shorter in SAA untreated ((4.4 ± 1.1) kb, n = 9) and recovering groups((5.8 ± 1.0) kb, n = 11) than control group ((9.2 ± 3.3) kb, P < 0.05). Telomere length of CD3(+)T cells shortened with TH/S decreasing (r = 0.564, P = 0.029). The mRNA expression of POT1 decreased in untreated SAA patients (0.16(0.02-0.29)) and over-expressed in recovering patients (1.17(0.82-1.86), P < 0.05). The mRNA expression of RAP1 was significantly higher in untreated patients (4.14 (1.93-6.92)) than that in recovering group (0.87 (0.30-1.73) ) and controls (0.62 (0.45-4.07) , both P < 0.05). Changes in telomere length and shelterin gene expression occur in CD3(+)T cells of SAA patients and may be correlated with disease seve...Continue Reading

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Mentioned in this Paper

Aplastic Anemia
T-Lymphocyte
Immunomagnetic Separation
Telomere Repeat Binding Proteins
TPP1 gene
Bone Marrow
TERF1 wt Allele
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
TINF2 protein, human

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