Telomere length measurement in mouse chromosomes by a modified Q-FISH method

Cytogenetic and Genome Research
H-P Wong, P Slijepcevic

Abstract

Telomeres are physical ends of mammalian chromosomes that dynamically change during the lifetime of a cell or organism. In order to understand mechanisms responsible for telomere dynamics, it is necessary to develop methods for accurate telomere length measurement. The most sensitive method for measuring telomere length in mouse chromosomes is quantitative fluorescence in situ hybridization (Q-FISH). The usual protocol for Q-FISH requires plasmids with variable numbers of telomeric repeats and fluorescence beads as calibration standards. Here, we describe a Q-FISH protocol in which two mouse lymphoma cell lines with well-defined telomere lengths are used as calibration standards. Using this protocol we demonstrate that reproducible results can be obtained in a set of four different mouse cell lines. This method can be adapted so that any pair of mammalian cell lines can serve as an internal calibration standard.

Citations

Apr 22, 2005·Chromosoma·Predrag Slijepcevic, Suliman Al-Wahiby
Jun 25, 2010·Molecular Human Reproduction·S TurnerG M Hartshorne
Mar 23, 2013·Molecular Human Reproduction·S Turner, G M Hartshorne
Nov 4, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Erik CabuyPredrag Slijepcevic
Feb 24, 2006·Mutation Research·Alejandro D Bolzán, Martha S Bianchi
Nov 11, 2010·Genetika·N S ZhdanovaJ-A Londono-Vallejo
Nov 27, 2020·Cell Reports·Jared J LuxtonSusan M Bailey
Dec 22, 2020·Plastic and Reconstructive Surgery·Jared J Luxton, Susan M Bailey
Apr 13, 2019·Science·Francine E Garrett-BakelmanFred W Turek

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