Telomere-telomere recombination provides an express pathway for telomere acquisition

Nature
S S Wang, V A Zakian

Abstract

DNA termini from Tetrahymena and Oxytricha, which bear C4A2 and C4A4 repeats respectively, can support telomere formation in Saccharomyces cerevisiae by serving as substrates for the addition of yeast telomeric C1-3A repeats. Previously, we showed that linear plasmids with 108 base pairs of C4A4 DNA (YLp108CA) efficiently acquired telomeres, whereas plasmids containing 28-64 base pairs of C4A4 DNA also promoted telomere formation, but with reduced efficiency. Although many of the C4A4 termini on these plasmids underwent recombination with a C4A2 terminus, the mechanism of telomere-telomere recombination was not established. We now report the sequence of the C4A4 ends from the linear plasmids. The results provide strong evidence for a novel recombination process involving a gene conversion event that requires little homology, occurs at or near the boundary of telomeric and nontelomeric DNA, and resembles the recombination process involved in bacteriophage T4 DNA replication.

Citations

Aug 26, 1998·Cancer·K YoshidaS Kitahara
Sep 1, 1996·Journal of Cellular Physiology·M B SmallK A Sethi
Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C W Greider
Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Holliday
Jul 1, 1994·Molecular Biology Reports·E DoreC Frontali
Dec 29, 1992·Biochimica Et Biophysica Acta·J S Coren, V M Vogt
Sep 1, 1990·Genetic Analysis, Techniques and Applications·J F Cheng, C L Smith
Mar 1, 1991·Mutation Research·C B Harley
Apr 1, 1993·Trends in Cell Biology·E GilsonS M Gasser
Feb 1, 1995·Trends in Genetics : TIG·J M Mason, H Biessmann
Dec 1, 1996·Current Opinion in Biotechnology·E RaymondD D Von Hoff
Dec 1, 1991·Current Opinion in Genetics & Development·E R Henderson, D D Larson
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T M Bryan, R R Reddel
Nov 6, 2007·Nature Cell Biology·Lin LiuDavid L Keefe
Dec 12, 2001·HIV Medicine·V TuckerM J Browning
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z LiuB K Tye
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P A KrukV A Bohr
Dec 11, 1990·Nucleic Acids Research·J A StarlingR C Allshire
Jun 25, 1992·Nucleic Acids Research·A J Lustig
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Christopher J WebbVirginia A Zakian
Jun 1, 1992·Journal of Medical Genetics·R HutchinsonL Voullaire
Jan 1, 1996·Annual Review of Genetics·V A Zakian
Feb 16, 2002·Biochemical and Biophysical Research Communications·Takeshi SudaYutaka Aoyagi
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M ZijlmansP M Lansdorp
Feb 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zeki TopcuMichael J McEachern
Apr 1, 1997·Current Opinion in Genetics & Development·T Smeal, L Guarente
May 1, 1994·Mutation Research·A Belmaaza, P Chartrand
Jan 1, 1993·Environmental and Molecular Mutagenesis·J P DayW F Morgan
Oct 23, 1997·The Journal of Cell Biology·P M Lansdorp
Apr 29, 1998·Cancer Investigation·P T Rowley
Nov 14, 1998·Annals of Medicine·V UrquidiS Goodison
Jan 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jozef NosekLubomir Tomaska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J Shampay, E H Blackburn
Proceedings of the National Academy of Sciences of the United States of America
H BiessmannJ M Mason
© 2021 Meta ULC. All rights reserved