Telomeres are partly shielded from ultraviolet-induced damage and proficient for nucleotide excision repair of photoproducts

Nature Communications
Dhvani ParikhPatricia L Opresko

Abstract

Ultraviolet light induces cyclobutane pyrimidine dimers (CPD) and pyrimidine(6-4)pyrimidone photoproducts, which interfere with DNA replication and transcription. Nucleotide excision repair (NER) removes these photoproducts, but whether NER functions at telomeres is unresolved. Here we use immunospot blotting to examine the efficiency of photoproduct formation and removal at telomeres purified from UVC irradiated cells at various recovery times. Telomeres exhibit approximately twofold fewer photoproducts compared with the bulk genome in cells, and telomere-binding protein TRF1 significantly reduces photoproduct formation in telomeric fragments in vitro. CPD removal from telomeres occurs 1.5-fold faster than the bulk genome, and is completed by 48 h. 6-4PP removal is rapidly completed by 6 h in both telomeres and the overall genome. A requirement for XPA protein indicates the mechanism of telomeric photoproduct removal is NER. These data provide new evidence that telomeres are partially protected from ultraviolet irradiation and that NER preserves telomere integrity.

References

Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P A KrukV A Bohr
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·J P HanishT de Lange
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D E SzymkowskiR D Wood
Nov 14, 1997·Genes & Development·W E WrightJ W Shay
Oct 16, 1999·The EMBO Journal·A BianchiT de Lange
Jun 3, 2000·Journal of Molecular Biology·J H YoonG P Pfeifer
Oct 6, 2000·Methods : a Companion to Methods in Enzymology·S Ayala-TorresB Van Houten
May 4, 2002·Nature Reviews. Genetics·Mark A Batzer, Prescott L Deininger
Aug 16, 2002·The Journal of Biological Chemistry·Patricia L OpreskoVilhelm A Bohr
Nov 26, 2002·Molecular and Cellular Biology·Timothy P HeffernanWilliam K Kaufmann
May 4, 2004·Molecular and Cellular Biology·Susanne SteinertWoodring E Wright
Feb 26, 2005·Nucleic Acids Research·Patricia L OpreskoVilhelm A Bohr
Sep 1, 2005·Journal of the American Geriatrics Society·Woodring E Wright, Jerry W Shay
Oct 28, 2005·The EMBO Journal·George A GarinisGijsbertus T J van der Horst
Dec 13, 2005·Molecular Biology of the Cell·Rebecca L TomlinsonMichael P Terns
Feb 4, 2006·Molecular Cell·Weihang ChaiWoodring E Wright
Feb 9, 2006·Chemical Reviews·Ludovic C J Gillet, Orlando D Schärer
Aug 19, 2007·Nature·Toren FinkelMaria A Blasco
Jan 30, 2008·Current Protocols in Cell Biology·Brittney-Shea HerbertWoodring E Wright
Aug 6, 2008·Annual Review of Genetics·Wilhelm Palm, Titia de Lange
Nov 22, 2008·Nature Reviews. Molecular Cell Biology·Philip C Hanawalt, Graciela Spivak
Jul 25, 2009·The EMBO Journal·Brian Luke, Joachim Lingner
Oct 9, 2009·The New England Journal of Medicine·Jan H J Hoeijmakers
Oct 30, 2009·The Journal of Biological Chemistry·Kaori K TakaiTitia de Lange
May 6, 2010·PLoS Genetics·Patrick J Rochette, Douglas E Brash
Jul 27, 2010·FEBS Letters·Titia de Lange
Jan 25, 2011·Nature Reviews. Cancer·Sabine S LangeRichard D Wood
Mar 19, 2011·Molecular Biotechnology·Tracy M Bryan, Peter Baumann
Dec 6, 2011·Nucleic Acids Research·Anaïs PouletMarie-Josèphe Giraud-Panis
Jan 6, 2012·The Journal of Investigative Dermatology·John J DiGiovanna, Kenneth H Kraemer
Mar 20, 2012·Nature Cell Biology·Marzia FumagalliFabrizio d'Adda di Fagagna

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Citations

Oct 1, 2015·DNA Repair·Pingping JiaWeihang Chai
May 29, 2016·DNA Repair·Elise FouquerelPatricia Opresko
May 25, 2016·Ageing Research Reviews·Patricia L Opresko, Jerry W Shay
Jun 15, 2016·Nucleic Acids Research·Jiangguo LinHong Wang
Nov 8, 2016·Nature Structural & Molecular Biology·Elise FouquerelPatricia L Opresko
Nov 20, 2016·Photochemistry and Photobiology·Elise Fouquerel, Patricia L Opresko
Aug 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wei QianBennett Van Houten
Feb 18, 2020·Cell Cycle·Stella Victorelli, João F Passos
Jul 5, 2018·International Journal of Molecular Sciences·Lina SakerSophie Bombard
Dec 25, 2017·Differentiation; Research in Biological Diversity·Wareed Ahmed, Joachim Lingner
Jun 3, 2021·Cells·Maria Sol Jacome Burbano, Eric Gilson
Mar 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Larissa Alexsandra da Silva Neto TrajanoAdenilson de Souza da Fonseca

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Methods Mentioned

BETA
immunoprecipitation
PCR
electrophoresis
X-ray
Assay

Software Mentioned

ImageJ
ImageQuant

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