Telomeric and rDNA silencing in Saccharomyces cerevisiae are dependent on a nuclear NAD(+) salvage pathway.

Genetics
Joseph J SandmeierJeffrey S Smith

Abstract

The Sir2 protein is an NAD(+)-dependent protein deacetylase that is required for silencing at the silent mating-type loci, telomeres, and the ribosomal DNA (rDNA). Mutations in the NAD(+) salvage gene NPT1 weaken all three forms of silencing and also cause a reduction in the intracellular NAD(+) level. We now show that mutation of a highly conserved histidine residue in Npt1p results in a silencing defect, indicating that Npt1p enzymatic activity is required for silencing. Deletion of another NAD(+) salvage pathway gene called PNC1 caused a less severe silencing defect and did not significantly reduce the intracellular NAD(+) concentration. However, silencing in the absence of PNC1 was completely dependent on the import of nicotinic acid from the growth medium. Deletion of a gene in the de novo NAD(+) synthesis pathway BNA1 resulted in a significant rDNA silencing defect only on medium deficient in nicotinic acid, an NAD(+) precursor. By immunofluorescence microscopy, Myc-tagged Bna1p was localized throughout the whole cell in an asynchronously growing population. In contrast, Myc-tagged Npt1p was highly concentrated in the nucleus in approximately 40% of the cells, indicating that NAD(+) salvage occurs in the nucleus in a sign...Continue Reading

References

Dec 30, 1992·Biochemical and Biophysical Research Communications·E BalducciP Natalini
Jan 2, 1992·Gene·T W ChristiansonP Hieter
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D E Gottschling
Apr 1, 1989·Journal of Bacteriology·U E ParkD R Hillyard
Dec 1, 1974·Journal of Cellular Physiology·M Rechsteiner, V Catanzarite
Mar 1, 1980·Journal of Cellular Physiology·T ManserF B Haugli
Jun 17, 1994·Science·S Loo, J Rine
Apr 1, 1993·Genes & Development·M BraunsteinJ R Broach
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D LaloP Thuriaux
Jan 1, 1995·Annual Review of Cell and Developmental Biology·S Loo, J Rine
Jun 1, 1996·Antimicrobial Agents and Chemotherapy·R FrothinghamK N Kreuzer
Jan 15, 1997·Genes & Development·J S Smith, J D Boeke
Jan 15, 1997·Genes & Development·M BrykM J Curcio
Nov 14, 1997·The EMBO Journal·C E FritzeR Easton Esposito
Mar 16, 2000·The EMBO Journal·T Kouzarides
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·J LandryR Sternglanz
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D E Sterner, S L Berger
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S SmithJ D Boeke
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·B E BernsteinS L Schreiber
Nov 30, 2000·Biochemical and Biophysical Research Communications·J LandryR Sternglanz
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·K G TannerJ M Denu
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Jun 21, 2002·Methods in Enzymology·Nurjana BachmanNancy L Craig

❮ Previous
Next ❯

Citations

Apr 5, 2003·Annual Review of Biochemistry·Laura N RuscheJasper Rine
Aug 19, 2007·Oncogene·A VaqueroD Reinberg
Jun 20, 2008·Genetics·Marguerite P AndersenDaniel E Gottschling
Aug 19, 2007·Annals of Medicine·Nassim Dali-YoucefJohan Auwerx
Aug 30, 2007·Molecular Microbiology·Biao MaBrendan P Cormack
Oct 3, 2003·The Journal of Biological Chemistry·Michael D JacksonJohn M Denu
Jul 23, 2004·The Journal of Biological Chemistry·Manning T SchmidtJohn M Denu
Oct 24, 2007·International Journal for Parasitology·Lakshminarayan M IyerL Aravind
Aug 6, 2008·The Journal of Biological Chemistry·Vitaly BalanGuri Tzivion
May 14, 2003·Fungal Genetics and Biology : FG & B·Delphine SicardRichard W Michelmore
Sep 24, 2004·The Journal of Biological Chemistry·Javier R RevolloShin-ichiro Imai
Oct 7, 2004·Molecular Carcinogenesis·Rakesh KumarChristopher J Barnes
Sep 26, 2002·The Journal of Biological Chemistry·Kevin J BittermanDavid A Sinclair
Jan 8, 2008·Nature Structural & Molecular Biology·Robert S Fuller
Nov 17, 2004·Genetics·Lisa L Freeman-CookPatricia Laurenson
Aug 29, 2018·Molecular & Cellular Proteomics : MCP·Sylvia VarlandThomas Arnesen
Mar 27, 2007·Archives of Biochemistry and Biophysics·Gang HuP John Hart
Jun 8, 2006·Annual Review of Biochemistry·Anthony A SauveJef D Boeke
Aug 27, 2005·Current Opinion in Chemical Biology·John M Denu
Feb 9, 2008·The Journal of Biological Chemistry·Peter A BelenkyCharles Brenner
Apr 7, 2007·Plant & Cell Physiology·Isabelle VaillantSylvette Tourmente
Sep 15, 2005·The FEBS Journal·Olivera GrubishaJohn M Denu
May 22, 2007·Ageing Research Reviews·Matt Kaeberlein, R Wilson Powers
Feb 1, 2005·Trends in Pharmacological Sciences·Marco Porcu, Alberto Chiarugi
Feb 22, 2005·Molecular Cell·Anthony A SauveIan M Willis
Feb 22, 2005·Journal of Cellular Biochemistry·Maria Rosaria Faraone-MennellaBenedetta Farina
Mar 26, 2015·Chemical Reviews·Hui Jing, Hening Lin
Mar 31, 2015·Experimental Biology and Medicine·Jessica SmithBrandt L Schneider
Jul 20, 2007·Aging Cell·Matt Kaeberlein, Brian K Kennedy
Jan 20, 2004·Molecular and Cellular Biology·Christopher M GalloJeffrey S Smith
Jan 27, 2004·Journal of Leukocyte Biology·Stephen W BuckJeffrey S Smith
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Kevin J BittermanDavid A Sinclair
Sep 16, 2003·Molecular and Cellular Biology·Antonio BedalovJulian A Simon
Aug 16, 2016·Genetics·Marc R Gartenberg, Jeffrey S Smith
Jul 19, 2002·Yeast

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