Telomeric fusions in cultured human fibroblasts as a source of genomic instability

Cancer Genetics and Cytogenetics
R RiboniC Mondello

Abstract

In a human fibroblast clone we studied the evolution, during culture propagation, of a dicentric chromosome consisting of the end-to-end association of the short arm of chromosome 5 and the long arm of chromosome 16. Dual-color fluorescence in situ hybridization (FISH) with painting probes allowed us to define the structure of a variety of derivative chromosomes and to identify the mechanisms by which they originated. Asymmetric interchanges involving the intercentromeric region of the dicentric, bridge-breakage-fusion events, or breaks followed by sister chromatid fusion, originate unstable hetero- or homodicentric chromosomes with deletion or duplication; breakages not followed by reunion, or intradicentric recombination, presumably originate stable rearranged monocentric chromosomes. The variety of the derivatives is extremely large because the observed events may involve any site of the intercentromeric region, although the majority of them occurs after a break in 16qh. The results of this investigation document the evolution through successive steps of a telomeric fusion, a chromosome anomaly frequently observed in tumor and senescent cells. They also demonstrate that in cultured cells of normal origin, starting with this ...Continue Reading

References

Jun 1, 1992·Cancer Genetics and Cytogenetics·J R SawyerF A Boop
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T RiedD C Ward
Oct 1, 1989·Trends in Genetics : TIG·N D Hastie, R C Allshire
Jan 1, 1995·Cytogenetics and Cell Genetics·T K PanditaC R Geard
Apr 1, 1995·Current Opinion in Genetics & Development·C B Harley, B Villeponteau
May 1, 1993·Genes, Chromosomes & Cancer·N Kokalj-VokacB Dutrillaux
Jan 1, 1995·Dermatologic Clinics·W C LambertM W Lambert
Jan 1, 1995·Cytogenetics and Cell Genetics·R AntonacciM Rocchi
Apr 1, 1994·Current Opinion in Genetics & Development·C W Greider
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M CounterC B Harley
Jun 1, 1993·Cancer Genetics and Cytogenetics·B K Vig, D Schroeter

❮ Previous
Next ❯

Citations

May 21, 2003·Breast Cancer Research and Treatment·Donna G Albertson
Jun 8, 2002·Molecular and Cellular Biology·Anthony W I LoJohn P Murnane
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·W S SaundersS M Gollin
Jan 1, 2011·Genetics Research International·Ruth N Mackinnon, Lynda J Campbell
Oct 30, 1998·In Vitro Cellular & Developmental Biology. Animal·C van Haaften-DayP Russell
May 20, 2003·Radiation Research·Jeffrey L SchwartzHoward Liber
Aug 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gabriela M Baerlocher, Peter M Lansdorp
Dec 12, 2003·European Journal of Haematology·Alejandra CottliarIrma Slavutsky
Sep 6, 2012·Journal of Cellular and Molecular Medicine·Rafael Díaz de la GuardiaPaola E Leone
Aug 25, 2004·Cancer Genetics and Cytogenetics·Sílvia CasasJorge Sierra
Jan 26, 2006·Cancer Genetics and Cytogenetics·Amber E AlsopPaul A W Edwards
Aug 17, 2005·Cancer Genetics and Cytogenetics·Déborah JametMarc De Braekeleer
Jan 9, 2014·Radiation Research·Constantinos G Broustas, Howard B Lieberman
Mar 30, 1999·Experimental Cell Research·C MondelloF Nuzzo
Jan 18, 2017·Nature Reviews. Molecular Cell Biology·John Maciejowski, Titia de Lange
Jul 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Chiara Mondello, A Ivana Scovassi
Jan 26, 2005·Journal of Dental Research·S C Reshmi, S M Gollin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.