Telomeric G-quadruplex-forming DNA fragments induce TLR9-mediated and LL-37-regulated invasion in breast cancer cells in vitro

Breast Cancer Research and Treatment
Johanna TuomelaKatri S Selander

Abstract

Toll-like receptor 9 (TLR9) is a cellular DNA-receptor widely expressed in cancers. We previously showed that synthetic and self-derived DNA fragments induce TLR9-mediated breast cancer cell invasion in vitro. We investigated here the invasive effects of two nuclease-resistant DNA fragments, a 9-mer hairpin, and a G-quadruplex DNA based on the human telomere sequence, both having native phosphodiester backbone. Cellular uptake of DNAs was investigated with immunofluorescence, invasion was studied with Matrigel-assays, and mRNA and protein expression were studied with qPCR and Western blotting and protease activity with zymograms. TLR9 expression was suppressed through siRNA. Although both DNAs induced TLR9-mediated changes in pro-invasive mRNA expression, only the telomeric G-quadruplex DNA significantly increased cellular invasion. This was inhibited with GM6001 and aprotinin, suggesting MMP- and serine protease mediation. Furthermore, complexing with LL-37, a cathelicidin-peptide present in breast cancers, increased 9-mer hairpin and G-quadruplex DNA uptake into the cancer cells. However, DNA/LL-37 complexes decreased invasion, as compared with DNA-treatment alone. Invasion studies were conducted also with DNA fragments isola...Continue Reading

References

Nov 1, 2002·The Journal of Biological Chemistry·Rafael RamírezMaría A Blasco
Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Misako MatsumotoTsukasa Seya
Jun 23, 2004·Trends in Immunology·Hermann Wagner
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cynthia A LeiferDavid M Segal
Dec 21, 2004·International Journal of Cancer. Journal International Du Cancer·Johan D HeilbornMona Ståhle
Jan 6, 2005·Respiratory Research·Daniel DroemannTorsten Goldmann
Jul 28, 2005·International Journal of Medical Microbiology : IJMM·Bernd SchmausserMatthias Eck
Jul 20, 2006·Molecular Cancer Research : MCR·Melinda A MerrellKatri S Selander
Aug 18, 2006·Nucleic Acids Research·Johanna Eddy, Nancy Maizels
Dec 16, 2006·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Mar 8, 2007·Critical Reviews in Immunology·Angela TrieuKatryn J Stacey
Mar 22, 2007·The Prostate·Joanna M IlvesaroKatri S Selander
May 3, 2007·Breast Disease·Naz Chaudary, Richard P Hill
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Seth B CoffeltAline B Scandurro
Oct 17, 2008·Molecular Cancer Research : MCR·Joanna M IlvesaroKatri S Selander
Feb 3, 2009·Breast Cancer Research : BCR·Günther WeberMona Ståhle
Mar 24, 2009·Molecular Carcinogenesis·Johanna Eddy, Nancy Maizels
Sep 12, 2009·Nucleic Acids Research·Himesh FernandoShankar Balasubramanian
Jan 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Plinio Hurtado, Chen Au Peh
Jan 8, 2010·The Prostate·Marja-Riitta VäisänenMarkku H Vaarala
Jan 1, 2009·Journal of Innate Immunity·Arja Jukkola-VuorinenKatri S Selander
Aug 4, 2010·Cell and Tissue Research·Raveendra KulkarniShayan Sharif
Dec 7, 2010·The Journal of Biological Chemistry·Zhongcheng ShiDekai Zhang
Sep 21, 2011·Journal of Experimental & Clinical Cancer Research : CR·Hanna RonkainenMarkku H Vaarala
Feb 23, 2012·Nucleic Acid Therapeutics·Arthur M Krieg
Mar 20, 2012·Cell Host & Microbe·Chih-Yuan ChiangSumit K Chanda
Aug 1, 2012·Breast Cancer Research and Treatment·Johanna TuomelaKatri S Selander
Aug 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Asa HidmarkAlexander H Dalpke
Oct 23, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Joonas H KauppilaKatri S Selander
Nov 1, 2012·Cellular Microbiology·Ian C BerkeYorgo Modis
Nov 12, 2013·Breast Cancer Research and Treatment·Johanna TuomelaKatri S Selander
Feb 4, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Johanna KorvalaTuula Salo
May 23, 2014·Frontiers in Immunology·Joonas H Kauppila, Katri S Selander

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.

© 2022 Meta ULC. All rights reserved