TeloPCR-seq: a high-throughput sequencing approach for telomeres

FEBS Letters
Henrietta W BennettMegan C King


We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of modular degenerate repeats that can be explained by variation in usage of the TER1 RNA template during reverse transcription. Taking advantage of this deep sequencing approach, we find that 'like' repeat modules are highly correlated within individual telomeres. Moreover, repeat module preference varies with telomere length, suggesting that existing repeats promote the incorporation of like repeats and/or that specific conformations of the telomerase holoenzyme efficiently and/or processively add repeats of like nature. After the loss of telomerase activity, this sequencing and analysis pipeline defines a population of telomeres with altered sequence content. This approach will be adaptable to study telomeric repeats in other organisms and also to interrogate repetitive sequences throughout the genome that are inaccessible to other sequencing methods.


Sep 1, 1991·Molecular and Cellular Biology·C W Greider
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Aug 15, 1997·Science·Toru M NakamuraThomas R Cech
May 19, 1998·Trends in Biochemical Sciences·Yasushi HiraokaE H Blackburn
Oct 17, 1998·Science·Toru M NakamuraThomas R Cech
May 25, 1999·Cell·J D GriffithTitia de Lange
Jul 25, 2000·Nucleic Acids Research·K FörstemannJoachim Lingner
Sep 13, 2000·Molecular Cell·Tracy M BryanThomas R Cech
Oct 5, 2001·Molecular and Cellular Biology·K Förstemann, Joachim Lingner
Apr 5, 2003·Current Biology : CB·Hans T H BeerninkJulia Promisel Cooper
Jan 6, 2004·Nucleic Acids Research·Dimitry Bosoy, Neal F Lue
Sep 17, 2005·Genes & Development·Titia de Lange
May 23, 2006·Current Opinion in Structural Biology·Carla A Theimer, Juli Feigon
Dec 25, 2007·Nature Structural & Molecular Biology·Christopher J Webb, Virginia A Zakian
Dec 25, 2007·Nature Structural & Molecular Biology·Jessica LeonardiPeter Baumann
Jun 7, 2008·Science·Tomoichiro MiyoshiFuyuki Ishikawa
Jun 25, 2008·The Journal of Biological Chemistry·Jessica A BoxPeter Baumann
Nov 22, 2008·Science·John EidStephen Turner
Jan 15, 2009·Genes & Development·Kazunori Tomita, Julia Promisel Cooper
Feb 14, 2009·The EMBO Journal·Bettina A MoserToru M Nakamura
Oct 23, 2009·Trends in Biochemical Sciences·Neal F Lue
Dec 8, 2009·Science·Titia de Lange
May 29, 2012·Molecular Cell·Pierre-Marie DehéJulia Promisel Cooper
Oct 30, 2012·Nature·Jayakrishnan NandakumarThomas R Cech
Dec 12, 2012·The Journal of Cell Biology·Dimitri ConomosHilda A Pickett
Jan 10, 2013·Nature Reviews. Molecular Cell Biology·Jayakrishnan Nandakumar, Thomas R Cech
Jul 5, 2013·Genome Biology·Richard J RobertsMichael C Schatz
Mar 25, 2014·Molecular Cell·Julien SoudetMaria Teresa Teixeira
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christopher J Webb, Virginia A Zakian

Related Concepts

DNA Sequence
Selfish DNA
Schizosaccharomyces pombe
Nested Polymerase Chain Reaction
Telomerase Catalytic Subunit
High-Throughput Nucleotide Sequencing
Nerve Degeneration
Reverse Transcription

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

Related Papers

BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Methods in Molecular Biology
Eliana B Gómez, Susan L Forsburg
Nature Structural & Molecular Biology
Jessica LeonardiPeter Baumann
© 2021 Meta ULC. All rights reserved