Telosome, a mammalian telomere-associated complex formed by multiple telomeric proteins.

The Journal of Biological Chemistry
Dan LiuZhou Songyang

Abstract

In mammalian cells, telomere-binding proteins TRF1 and TRF2 play crucial roles in telomere biology. They interact with several other telomere regulators including TIN2, PTOP, POT1, and RAP1 to ensure proper maintenance of telomeres. TRF1 and TRF2 are believed to exert distinct functions. TRF1 forms a complex with TIN2, PTOP, and POT1 and regulates telomere length, whereas TRF2 mediates t-loop formation and end protection. However, whether cross-talk occurs between the TRF1 and TRF2 complexes and how the signals from these complexes are integrated for telomere maintenance remain to be elucidated. Through gel filtration and co-immunoprecipitation experiments, we found that TRF1 and TRF2 are in fact subunits of a telomere-associated high molecular weight complex (telosome) that also contains POT1, PTOP, RAP1, and TIN2. We demonstrated that the TRF1-interacting protein TIN2 binds TRF2 directly and in vivo, thereby bridging TRF2 to TRF1. Consistent with this multi-protein telosome model, stripping TRF1 off the telomeres by expressing tankyrase reduced telomere recruitment of not only TIN2 but also TRF2. These results help to unify previous observations and suggest that telomere maintenance depends on the multi-subunit telosome.

References

Feb 1, 1992·Genes & Development·J H WrightV A Zakian
Feb 20, 1997·Nature·B van Steensel, T de Lange
Feb 26, 1998·Cell·B van SteenselT de Lange
Jul 23, 1998·Cell·V V OgryzkoY Nakatani
Nov 20, 1998·Science·S SmithT de Lange
May 25, 1999·Cell·J D GriffithT de Lange
Dec 2, 1999·Nature Genetics·S H KimJ Campisi
Feb 12, 2000·Molecular and Cellular Biology·A SmogorzewskaT de Lange
Nov 9, 2000·Current Biology : CB·S Smith, T de Lange
May 10, 2001·Current Opinion in Cell Biology·K DubranaS M Gasser
Feb 19, 2002·Oncogene·Sahn-ho Kim ShJudith Campisi
Feb 19, 2002·Oncogene·Titia de Lange
Mar 30, 2002·Science·Jan KarlsederTitia de Lange
May 28, 2003·Nature·Diego Loayza, Titia De Lange
Jun 5, 2003·Current Biology : CB·Lorel M ColginRoger R Reddel
Jun 5, 2003·Genes & Development·William ChangSusan Smith
Aug 29, 2003·Nature·Mauro SerafiniAlan Crozier
Oct 16, 2003·The Journal of Biological Chemistry·Tomohiko IwanoYoichi Shinkai
Nov 20, 2003·Cellular and Molecular Life Sciences : CMLS·C Wei, M Price
Apr 22, 2004·The Journal of Biological Chemistry·Matthew S O'ConnorZhou Songyang
May 11, 2004·Nature Genetics·Jeffrey Zheng-Sheng Ye, Titia de Lange
Jun 8, 2004·Nature Cell Biology·Dan LiuZhou Songyang
Jul 3, 2004·Genes & Development·Jeffrey Zheng-Sheng YeTitia de Lange
Aug 5, 2004·The Journal of Biological Chemistry·Sahn-ho KimJudith Campisi
Aug 19, 2004·The Journal of Biological Chemistry·Jeffrey Zheng-Sheng YeTitia de Lange

❮ Previous
Next ❯

Citations

Jul 14, 2005·Chromosoma·Giovanni CenciMaurizio Gatti
Jun 3, 2006·Chromosoma·Nele Hug, Joachim Lingner
Nov 19, 2010·Molecular Biology Reports·Aneta WojtylaBlazej Rubis
Jun 15, 2011·International Journal of Hematology·Zhenyu JuTao Cheng
Nov 4, 2008·Journal of the American Chemical Society·Raphaël RodriguezShankar Balasubramanian
Mar 23, 2011·Cell Research·Dong YangZhou Songyang
Jun 22, 2005·Nature Cell Biology·Giulia B Celli, Titia de Lange
Sep 19, 2007·Nature Chemical Biology·Maria A Blasco
Oct 27, 2010·Nature Communications·Isabel López de SilanesMaria A Blasco
Jun 30, 2011·Nature Communications·Megan McKerlie, Xu-Dong Zhu
Jun 6, 2013·Nature Communications·Ralph P SchneiderMaria A Blasco
Nov 26, 2013·Nature Communications·Larissa GrolimundJoachim Lingner
Feb 8, 2008·Nature Reviews. Cancer·Calvin B Harley
May 27, 2008·Nature Reviews. Cancer·Yibin DengSandy Chang
Mar 17, 2007·Nature Reviews. Genetics·María A Blasco
Jan 10, 2013·Nature Reviews. Molecular Cell Biology·Jayakrishnan Nandakumar, Thomas R Cech
Mar 17, 2009·Nature Structural & Molecular Biology·Hyeung KimZhou Songyang
Jan 13, 2009·Oncogene·A Saharia, S A Stewart
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Peisu ZhangMark P Mattson
Oct 30, 2009·The Journal of Biological Chemistry·Kaori K TakaiTitia de Lange
Oct 9, 2009·The Journal of Biological Chemistry·Gregory SowdPatricia L Opresko
Oct 21, 2010·The Journal of Biological Chemistry·Ji Hoon LeeIn Kwon Chung
Sep 10, 2010·The Journal of Biological Chemistry·Tanya L WilliamsElizabeth H Blackburn
May 4, 2011·The Journal of Biological Chemistry·Dong YangZhou Songyang
Oct 23, 2012·The Journal of Biological Chemistry·N Özlem Arat, Jack D Griffith
Jul 3, 2008·The Journal of Biological Chemistry·Brian D Freibaum, Christopher M Counter
Jul 1, 2008·The Journal of Biological Chemistry·Keiji OkamotoYoichi Shinkai
Jan 24, 2009·The Journal of Biological Chemistry·Yoon Ra Her, In Kwon Chung
Jun 4, 2010·Antioxidants & Redox Signaling·Ursula Muñoz-Najar, John M Sedivy
Mar 27, 2009·Molecular Biology of the Cell·Carol CooleyCiaran G Morrison
Nov 17, 2007·Nucleic Acids Research·Robert M Brosh, Vilhelm A Bohr
Oct 5, 2007·Nucleic Acids Research·Sascha Beneke, Alexander Bürkle
Oct 7, 2008·Nucleic Acids Research·Sascha BenekeAlexander Bürkle
Dec 2, 2008·Nucleic Acids Research·David C ZappullaDeborah S Wuttke
Jan 9, 2010·Nucleic Acids Research·Sabrina PisanoStefano Cacchione
Jun 18, 2009·Nucleic Acids Research·Asmaa M BakerTerace M Fletcher
Dec 6, 2011·Nucleic Acids Research·Anaïs PouletMarie-Josèphe Giraud-Panis
Aug 20, 2011·Nucleic Acids Research·Kyung H ChoiMichel M Ouellette
Jan 12, 2013·Nucleic Acids Research·Yoon Ra Her, In Kwon Chung
Aug 21, 2012·Nucleic Acids Research·Nicole L BatenburgXu-Dong Zhu
Jan 17, 2008·Genes & Development·Maria Pia Longhese
Aug 26, 2011·Genes & Development·Silvia CanudasSusan Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.