Dec 25, 2013

TeloTool: a new tool for telomere length measurement from terminal restriction fragment analysis with improved probe intensity correction

Nucleic Acids Research
Janett GöhringKarel Riha

Abstract

Telomeres comprise the protective caps of natural chromosome ends and function in the suppression of DNA damage signaling and cellular senescence. Therefore, techniques used to determine telomere length are important in a number of studies, ranging from those investigating telomeric structure to effects on human disease. Terminal restriction fragment (TRF) analysis has for a long time shown to be one of the most accurate methods for quantification of absolute telomere length and range from a number of species. As this technique centers on standard Southern blotting, telomeric DNA is observed on resulting autoradiograms as a heterogeneous smear. Methods to accurately determine telomere length from telomeric smears have proven problematic, and no reliable technique has been suggested to obtain mean telomere length values. Here, we present TeloTool, a new program allowing thorough statistical analysis of TRF data. Using this new method, a number of methodical biases are removed from previously stated techniques, including assumptions based on probe intensity corrections. This program provides a standardized mean for quick and reliable extraction of quantitative data from TRF autoradiograms; its wide application will allow accurate...Continue Reading

  • References48
  • Citations20

Mentioned in this Paper

Cell Aging
Telomere Lengthening
Southern Blot Result
Computer Programs and Programming
Visual Suppression
Smear Sample
Telomere
Chromosomes
Metabolic Suppression
DNA Restriction Enzymes

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