TEM and SHV Genes in Klebsiella pneumoniae Isolated from Cockroaches and Their Antimicrobial Resistance Pattern

Osong Public Health and Research Perspectives
Abbas DoostiHamidreza Kabiri

Abstract

Klebsiella pneumoniae is a gram-negative rod bacterium, a known cause of community-acquired bacterial pneumonia and is an important hospital-acquired pathogen that causes severe morbidity and mortality. The aim of this study was to identify the TEM and SHV genes in K. pneumoniae isolated from cockroaches obtained from hospitals. In this study, 250 cockroaches were collected from different hospitals in the province of Chaharmahal Va Bakhtiari, which is located in southwest Iran. The samples were examined for the presence of K. pneumoniae by plating onto a combination of culture media, and the antimicrobial susceptibility patterns of isolated K. pneumoniae from samples were evaluated using the disk diffusion test. In addition, from the culture, genomic bacterial DNA was extracted, and sequence-specific targets (TEM and SHV genes) were amplified using the polymerase chain reaction (PCR) method. Out of 250 cockroach samples collected from various hospitals, 179 samples (71.60%) were positive for K. pneumoniae. PCR reaction was performed using specific oligonucleotide primers (TEM-F, TEM-R and SHV-F, SHV-R) for the amplification of each gene, and amplified products were visualized on 1% agarose gel electrophoresis. Of all the specim...Continue Reading

References

Jan 1, 1984·British Medical Bulletin·A A Medeiros
Oct 1, 1995·Clinical Microbiology Reviews·D M Livermore
Nov 3, 2005·Molecular Microbiology·Matthew S LawlorVirginia L Miller
Aug 19, 2009·Antimicrobial Agents and Chemotherapy·Michael R Mulvey, David A Boyd
Oct 6, 2010·Antimicrobial Agents and Chemotherapy·Thérèse StachyraMichael T Black

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPSS
MS Excel

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