Temozolomide combined with irinotecan regresses a cisplatinum-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) precision-oncology mouse model

Oncotarget
Kentaro IgarashiRobert M Hoffman

Abstract

Relapsed osteosarcoma is a recalcitrant tumor. A patient's cisplatinum (CDDP)-resistant relapsed osteosarcoma lung metastasis was previously established orthotopically in the distal femur of mice to establish a patient-derived orthotopic xenograft (PDOX) model. In the present study, the PDOX models were randomized into the following groups when tumor volume reached 100 mm3: G1, control without treatment; G2, CDDP (6 mg/kg, intraperitoneal (i.p.) injection, weekly, for 2 weeks); gemcitabine (GEM) (100 mg/kg, i.p., weekly, for 2 weeks) combined with docetaxel (DOC) (20 mg/kg, i.p., once); temozolomide (TEM) (25 mg/kg, p.o., daily, for 2 weeks) combined with irinotecan (IRN) (4 mg/kg i.p., daily for 2 weeks). Tumor size and body weight were measured with calipers and a digital balance twice a week. After 2 weeks, all treatments significantly inhibited tumor growth except CDDP compared to the untreated control: CDDP: p = 0.093; GEM+DOC: p = 0.0002, TEM+IRN: p < 0.0001. TEM combined with IRN was significantly more effective than either CDDP (p = 0.0001) or GEM combined with DOC (p = 0.0003) and significantly regressed the tumor volume compared to day 0 (p = 0.003). Thus the PDOX model precisely identified the combination of TEM-IRN ...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·X FuR M Hoffman
Jul 30, 1992·International Journal of Cancer. Journal International Du Cancer·X WangR M Hoffman
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·X Y FuR M Hoffman
Jul 17, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P A MeyersJ H Healey
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G BacciM DePaolis
Oct 2, 2003·British Journal of Cancer·M V Blagosklonny
Dec 18, 2003·Drug Discovery Today·Mikhail V Blagosklonny
Dec 14, 2004·The Journal of Bone and Joint Surgery. American Volume·D Luis MuscoloMaximiliano Ranalletta
Nov 1, 2005·Cell Cycle·Mikhail V Blagosklonny
Dec 17, 2005·Leukemia·M V Blagosklonny
Feb 26, 2009·Natural Product Reports·Carmen Cuevas, Andrés Francesch
Jan 22, 2013·Pediatric Blood & Cancer·Stephen T KeirMalcolm A Smith
Feb 16, 2013·Cancer Cell·Giovanni GermanoPaola Allavena
Mar 3, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Axel Le CesneUNKNOWN French Sarcoma Group

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