Temozolomide, quercetin and cell death in the MOGGCCM astrocytoma cell line

Chemico-biological Interactions
Joanna Jakubowicz-GilWojciech Rzeski

Abstract

The aim of the present study was to investigate the effect of Temozolomide (an alkylating chemotherapeutic agent) and quercetin (natural flavonoid) on cell death in the human astrocytoma cell line MOGGCCM (WHO grade III). Our results indicate that Temozolomide induces autophagy, while quercetin promotes severe necrosis in the cell line in a manner dependent on the drug concentration. We demonstrated for the first time that combinations of both drugs were much more effective in programmed cell death induction in glioma cells. At a low (5muM) drug concentration, quercetin potentiated a pro-autophagic effect of Temozolomide, while after treatment with a higher drug concentration (30muM), autophagy switched to apoptosis. Temozolomide attenuated the toxic effect of quercetin. Apoptosis was mediated by the mitochondrial pathway and the activation of caspase 3 and cytochrome C release, but no changes in caspase 8 expression was observed. It was accompanied by decreased mitochondrial membrane potential and inhibition of Hsp27 and Hsp72 expression. Autophagy was correlated with an increased level of LC3II. Temozolomide and quercetin also inhibited migratory phenotype of MOGGCCM cells and changed the nuclei morphology from a circular to ...Continue Reading

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Citations

Nov 15, 2012·Analytical and Bioanalytical Chemistry·Ilanna C LopesAna Maria Oliveira-Brett
Apr 13, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Joanna Jakubowicz-GilWojciech Rzeski
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Dec 25, 2013·Neurotoxicity Research·Joanna Jakubowicz-GilWojciech Rzeski
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Dec 28, 2019·BioFactors·Erfan TavanaAmirhossein Sahebkar
Nov 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danuta SugierRadosław Kowalski
Mar 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Piotr SugierDariusz Dziki
Jan 18, 2018·Molecular Nutrition & Food Research·Sarah Tomas-HernándezMiquel Mulero
Nov 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joanna Sumorek-WiadroJoanna Jakubowicz-Gil
Jun 3, 2021·International Journal of Molecular Sciences·Adrian ZającJoanna Jakubowicz-Gil
Jun 3, 2021·Cancers·Aleksandra MaciejczykJoanna Jakubowicz-Gil

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