Temperature- and Composition-Dependent DNA Condensation by Thermosensitive Block Copolymers

ACS Omega
Satyagopal SahooDibakar Dhara

Abstract

Successful intracellular delivery of genes requires an efficient carrier, as genes by themselves cannot diffuse across cell membranes. Because of the toxicity and immunogenicity of viral vectors, nonviral vectors are gaining tremendous interest in research. In this work, we have investigated the temperature-dependent DNA condensation efficiency of various compositions of a thermosensitive block copolymer viz., poly(N-isopropylacrylamide)-b-poly(2-(diethylamino)ethyl methacrylate) (PNIPA-b-PDMAEMA). Three different copolymer compositions of varying molecular weights were successfully synthesized via the RAFT polymerization technique. Steady-state fluorescence and circular dichroism (CD) spectroscopies, dynamic light scattering (DLS) and zeta potential measurements, agarose gel electrophoresis, and atomic force microscopy techniques were utilized to study the interaction of the copolymers with DNA at temperatures above and below the critical aggregation temperature (CAT). All these experiments revealed that, above the CAT, there was formation of highly stable and tight polymer-DNA complexes (polyplexes). The size of polyplexes was dependent on the temperature up to a certain charge ratio, as determined by the DLS results. The res...Continue Reading

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Citations

Mar 23, 2021·Biomacromolecules·Christopher C PerryJamie R Milligan
Oct 16, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Satyagopal SahooDibakar Dhara

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Methods Mentioned

BETA
transfection
nuclear magnetic resonance
atomic force microscopy
Fluorescence
electrophoresis
AFM
transmission electron microscopy
light scattering
NMR

Software Mentioned

Agilent PicoView

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