Temperature and driving force dependence of the folding rate of reduced horse heart cytochrome c

Biochemistry
T Pascher

Abstract

Utilizing the stability difference between the ferro and ferri forms of horse heart cytochrome c (cyt c), folding of reduced cyt c was triggered by laser-induced reduction of unfolded oxidized cyt c. Measurements were made of the kinetics of the main folding phase (1 ms-10 s) in which collapsed reduced cyt c transforms to the native conformation. The folding rates were studied extensively as a function of temperature (5-75 degrees C) and guanidine hydrochloride (GdnHCl) concentration (1.6-4.9 M). At constant [GdnHCl], the Arrhenius plot of the folding rate constant (k) is nonlinear. At temperatures above 40 degrees C, the decrease in protein stability counteracts the expected increase in folding rate. Introducing free energy (DeltaG), derived from protein stability data, into the Eyring and Arrhenius equations leads to: ln k = ln(k(b)T/h) + DeltaS()/R - DeltaH()/RT - theta(m)DeltaG/RT = ln A - E(a)/RT - theta(m)DeltaG/RT, where theta(m) is the ratio between the denaturant dependence of the folding rate and the stability. By using this equation at constant DeltaG [or constant equilibrium constant (K)], linear Arrhenius plots are obtained. For the main folding phase of reduced cyt c, a positive DeltaS() is obtained indicating tha...Continue Reading

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Citations

Feb 25, 2009·European Biophysics Journal : EBJ·G M ArtmannA Temiz Artmann
Aug 14, 2010·Journal of the American Chemical Society·Suman Kalyan PalVilly Sundström
May 27, 2009·International Journal of Molecular Sciences·Robert A GoldbeckDavid S Kliger
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Feb 17, 2006·Physical Chemistry Chemical Physics : PCCP·Masahide Terazima
Jun 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tae Wu KimHyotcherl Ihee
Oct 30, 2007·The Journal of Physical Chemistry. a·Alexander N TarnovskyTorbjörn Pascher
Jan 19, 2006·Journal of the American Chemical Society·Tetsunari KimuraKoichiro Ishimori
Jun 15, 2007·Journal of the American Chemical Society·Swati DeVilly Sundström
Nov 15, 2002·Journal of the American Chemical Society·Jie PanTomás Polívka

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