Temperature and feeding induce tissue level changes in autotrophic and heterotrophic nutrient allocation in the coral symbiosis - A NanoSIMS study

Scientific Reports
Thomas KruegerAnders Meibom

Abstract

Corals access inorganic seawater nutrients through their autotrophic endosymbiotic dinoflagellates, but also capture planktonic prey through heterotrophic feeding. Correlating NanoSIMS and TEM imaging, we visualized and quantified the subcellular fate of autotrophic and heterotrophic C and N in the coral Stylophora pistillata using stable isotopes. Six scenarios were compared after 6 h: autotrophic pulse (13C-bicarbonate, 15N-nitrate) in either unfed or regularly fed corals, and heterotrophic pulse (13C-, 15N-labelled brine shrimps) in regularly fed corals; each at ambient and elevated temperature. Host assimilation of photosynthates was similar under fed and unfed conditions, but symbionts assimilated 10% more C in fed corals. Photoautotrophic C was primarily channelled into host lipid bodies, whereas heterotrophic C and N were generally co-allocated to the tissue. Food-derived label was detected in some subcellular structures associated with the remobilisation of host lipid stores. While heterotrophic input generally exceeded autotrophic input, it was more negatively affected by elevated temperature. The reduced input from both modes of nutrition at elevated temperature was accompanied by a shift in the partitioning of C and ...Continue Reading

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Citations

Jun 1, 2019·Integrative and Comparative Biology·Virginia M Weis
Jul 13, 2019·Annual Review of Marine Science·Xavier Mayali
Mar 5, 2020·Proceedings. Biological Sciences·Thomas KruegerAnders Meibom
Jun 18, 2020·Proceedings. Biological Sciences·Charlotte LeKieffreAnders Meibom
Sep 20, 2019·Ecology and Evolution·Maha J CziesielskiManuel Aranda
Jun 30, 2019·The ISME Journal·Valentine MeunierFanny Houlbrèque
Nov 17, 2020·Environmental Microbiology Reports·Tomihiko HiguchiYuji Sano
Dec 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Peter MojzešAnya Salih
Dec 17, 2020·Proceedings. Biological Sciences·Niclas Heidelberg LyndbyAnders Meibom
Jan 28, 2021·Proceedings of the National Academy of Sciences of the United States of America·Nils RädeckerChristian R Voolstra
Jun 5, 2021·Royal Society Open Science·Ariana S HuffmyerRuth D Gates

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Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

IMAGE
NanoSIMS

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