Temperature and pressure effects on structural and conformational properties of POPC/SM/cholesterol model raft mixtures--a FT-IR, SAXS, DSC, PPC and Laurdan fluorescence spectroscopy study

Biochimica Et Biophysica Acta
Chiara NicoliniRoland Winter

Abstract

We report on the effects of temperature and pressure on the structure, conformation and phase behavior of aqueous dispersions of the model lipid "raft" mixture palmitoyloleoylphosphatidylcholine (POPC)/bovine brain sphingomyelin (SM)/cholesterol (Chol) (1:1:1). We investigated interchain interactions, hydrogen bonding, conformational and structural properties as well as phase transformations of this system using Fourier transform-infrared (FT-IR) spectroscopy, small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) coupled with pressure perturbation calorimetry (PPC), and Laurdan fluorescence spectroscopy. The IR spectral parameters in combination with the scattering patterns from the SAXS measurements were used to detect structural and conformational transformations upon changes of pressure up to 7-9 kbar and temperature in the range from 1 to about 80 degrees C. The generalized polarization function (GP) values, obtained from the Laurdan fluorescence spectroscopy studies also reveal temperature and pressure dependent phase changes. DSC and PPC were used to detect thermodynamic properties accompanying the temperature-dependent phase changes. In combination with literature fluorescence spectroscopy and micr...Continue Reading

References

Mar 1, 1991·Chemistry and Physics of Lipids·H H Mantsch, R N McElhaney
Jun 1, 1990·Biophysical Journal·T ParasassiE Gratton
Aug 23, 1988·Biochemistry·K Simons, G van Meer
Nov 27, 1987·Biochimica Et Biophysica Acta·J H IpsenM J Zuckermann
Jan 1, 1984·Progress in Biophysics and Molecular Biology·A Cooper
Mar 1, 1994·Chemistry and Physics of Lipids·A NilssonG Lindblom
Feb 28, 2002·Biophysical Journal·Heiko Heerklotz, Joachim Seelig
Nov 5, 2002·Biophysical Journal·H Heerklotz
Dec 18, 2002·Physical Review Letters·Sarah L Veatch, Sarah L Keller
Jan 14, 2003·Biophysical Journal·Sarah L Veatch, Sarah L Keller
Jan 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Michael Edidin
May 9, 2003·The Journal of Biological Chemistry·Nicoletta KahyaPetra Schwille
Sep 26, 2003·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
Nov 19, 2003·Cell·Sean Munro
May 14, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Revanur Ravindra, Roland Winter
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
Aug 6, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S Scarlata

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Citations

Oct 22, 2008·Applied and Environmental Microbiology·Kai LinkeRudi F Vogel
May 6, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Duygu BilgeFeride Severcan
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Jan 25, 2011·Biophysical Chemistry·Yong ZhaiRoland Winter
Aug 11, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tsubasa Miyoshi, Satoru Kato
Aug 30, 2008·Chemistry and Physics of Lipids·Rodrigo F M de AlmeidaManuel Prieto
Dec 22, 2010·Chemistry and Physics of Lipids·Nicholas J BrooksJohn M Seddon
Jul 27, 2012·Molecular Membrane Biology·Jesús PradesFrancisca Barceló
Jun 16, 2012·Biochimica Et Biophysica Acta·Andreas Max ErnstBritta Brügger
Jan 27, 2015·Biochimica Et Biophysica Acta·Mattia SacchiAndrea Alessandrini
Apr 13, 2010·Biochimica Et Biophysica Acta·Yann RocheJean-Marie Perrier-Cornet
Nov 5, 2016·Biochimica Et Biophysica Acta·Evan M Kerek, Elmar J Prenner
Oct 28, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Ana Rute NevesSalette Reis
Apr 25, 2015·Chemical Communications : Chem Comm·Nicola L C McCarthyNicholas J Brooks
Mar 2, 2016·Biochimica Et Biophysica Acta·M RamezanpourD P Tieleman
Feb 24, 2015·Physical Chemistry Chemical Physics : PCCP·Janine SeeligerRoland Winter
Sep 7, 2018·BioMed Research International·Aswin T SrivatsavShobhna Kapoor
Dec 7, 2017·Biophysical Journal·Scott P RayermannSarah L Keller
Aug 21, 2008·The Journal of Physical Chemistry. B·Alessio AusiliJuan C Gómez-Fernández
Oct 15, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ashkan RahmaniMichael R Morrow
Apr 16, 2014·The Journal of Physical Chemistry. B·Eva ZupancicLiana C Silva
Mar 24, 2011·The Journal of Physical Chemistry. B·Heather A Pillman, G J Blanchard
Aug 28, 2021·International Journal of Molecular Sciences·Mayra Quemé-PeñaTamás Beke-Somfai
Aug 5, 2020·Biochimica Et Biophysica Acta. Biomembranes·Marius HerzogRoland Winter

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