Temperature dependence of the thrombin-catalyzed proteolysis of prothrombin

Biophysical Chemistry
Fang ShiCraig M Jackson

Abstract

Measurement of the temperature-dependence of thrombin-catalyzed cleavage of the Arg(155)-Ser(156) and Arg(284)-Thr(285) peptide bonds in prothrombin and prothrombin-derived substrates has yielded Arrhenius parameters that are far too large for classical mechanistic interpretation in terms of a simple hydrolytic reaction. Such a difference from the kinetic behavior exhibited in trypsin- and chymotrypsin-catalyzed proteolysis of peptide bonds is attributed to contributions by enzyme exosite interactions as well as enzyme conformational equilibria to the magnitudes of the experimentally determined Arrhenius parameters. Although the pre-exponential factor and the energy of activation deduced from the temperature-dependence of rate constants for proteolysis by thrombin cannot be accorded the usual mechanistic significance, their evaluation serves a valuable role by highlighting the existence of contributions other than those emanating from simple peptide hydrolysis to the kinetics of proteolysis by thrombin and presumably other enzymes of the blood coagulation system.

References

Dec 1, 1992·Biochemistry·C M Wells, E Di Cera
Oct 15, 1991·Biochemistry·S R StoneJ Hofsteenge
Aug 22, 1989·Biochemistry·M Soriano-GarciaE Skrzypczak-Jankun
Aug 20, 1988·Journal of Molecular Biology·A TulinskyE Skrzypczak-Jankun
Jan 1, 1986·Annals of the New York Academy of Sciences·J W Fenton
Jan 1, 1980·Annual Review of Biochemistry·C M Jackson, Y Nemerson
Jan 1, 1981·Annals of the New York Academy of Sciences·J W Fenton
Apr 9, 1996·Biochemistry·A Vindigni, E Di Cera
Jul 9, 1996·Biochemistry·E R Guinto, E Di Cera
Jul 16, 1996·Biochemistry·V ArocasM Jandrot-Perrus
Jun 3, 1997·Biochemistry·A VindigniE Di Cera

❮ Previous
Next ❯

Citations

Sep 14, 2010·Biochimica Et Biophysica Acta·Ismael Bustos-JaimesAmelia Farrés
May 6, 2008·Biochimica Et Biophysica Acta·Matteo MozzicafreddoMauro Angeletti
May 13, 2006·Biochimie·M MozzicafreddoM Angeletti
Nov 14, 2008·Journal of Molecular Recognition : JMR·Massimiliano CuccioloniMauro Angeletti
Feb 5, 2005·Analytical Biochemistry·Donald J Winzor, Craig M Jackson
Dec 18, 2014·Molecular & Cellular Proteomics : MCP·Fabio SabinoUlrich auf dem Keller
Aug 10, 2006·Journal of Molecular Recognition : JMR·Donald J Winzor, Craig M Jackson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.