Mar 16, 1976

Temperature-dependent change in rate-limiting step of the magnesium-stimulated ITPase of myosin

European Journal of Biochemistry
T Hozumi

Abstract

The effects of temperature on Mg-ITPase activity of heavy meromyosin and myosin subfragment 1 were measured in 0.1 M KC1. The initial burst of Pi liberation was one mol per mol of heavy meromyosin or two mol of myosin subfragment 1, i.e. one mol per two mol of myosin active sites, at 20 degrees C. However, it was almost zero mol below 8degrees C. Effects of KC1 concentration and pH on ITPase activity of heavy meromyosin at 20 degrees C were different from those below 8 degrees C, suggesting that the rate-limiting step in the Mg-ITP hydrolysis of myosin depends on temperature. The effect of temperature on the actin activation of heavy meromyosin Mg-ITPase was analyzed by measuring the temperature dependence of double-reciprocal plots of ITPase activity against actin concentration. The extent of actin activation was larger at low temperture. The results presented in this paper might be explained by assuming the existence of two kinds of active sites on a myosin molecule.

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Mentioned in this Paper

Thermodynamics
Magnesium Measurement
Phosphoric diester hydrolase
Actins
ITP Catabolic Process
Inosine triphosphatase
Slow-K
Actomyosin Adenosinetriphosphatase
Myosin ATPase
ITPA

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