Temperature dependent expression of an acid phosphatase by Bordetella bronchiseptica: role in intracellular survival

Microbial Pathogenesis
G S ChhatwalC A Guzman

Abstract

Bordetella bronchiseptica has the ability to invade and survive intracellularly. This potential to survive for extended periods within eukaryotic cells might play an important role in the pathogenesis of the infections caused by this microorganism. The bacterial factors involved in this process, however, have not yet been determined. In this study we have identified an acid phosphatase produced by B. bronchiseptica, but not by other Bordetella spp. The expression of this enzyme was demonstrated to be strictly regulated by temperature (optimal expression at 30 degrees C) and seems to be partially repressed by the product of the bvg locus. The enzyme was localized in the cytoplasmic fraction, the optimal activity was observed at pH 5.5, and the apparent molecular mass obtained by zymogram was 40 kDa. To further investigate the pathogenic role of this enzyme, mutants lacking acid phosphatase activity were obtained from both bvg-positive and bvg-negative parental strains using minitransposons. The growth pattern of these recombinant clones in vitro was similar to the parent strains, however, the tested clones exhibited a significant reduction (P < or = 0.05) in their intracellular survival ability. This newly described acid phospha...Continue Reading

References

Nov 1, 1992·Infection and Immunity·R L FriedmanD E Yocum
Mar 1, 1992·Microbiological Reviews·J N DowlingR H Glew
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H R Masure
Feb 1, 1992·Infection and Immunity·D T BeattieJ J Mekalanos
Nov 1, 1989·Journal of Bacteriology·A R Melton, A A Weiss
Jul 1, 1989·Journal of Bacteriology·R Gross, R Rappuoli
Sep 1, 1989·Infection and Immunity·C A EwanowichM S Peppler
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B AricóR Rappuoli
Dec 1, 1989·Vaccine·A RobinsonL I Irons
Dec 1, 1988·Microbiological Reviews·R H GlewA T Remaley
Oct 1, 1970·Journal of General Microbiology·D W Stainer, M J Scholte
Jan 1, 1984·Infection and Immunity·A A Weiss, S Falkow
Dec 1, 1984·Journal of Bacteriology·G F AmesS Kustu
Dec 1, 1980·Microbiological Reviews·R A Goodnow
Dec 1, 1994·Infection and Immunity·C A GuzmanK N Timmis
Jul 1, 1994·Infection and Immunity·H SchipperR Gross
Feb 1, 1993·Microbial Pathogenesis·P H SavelkoulW Gaastra
Dec 1, 1993·The Journal of Experimental Medicine·P PagliaP Ricciardi-Castagnoli
Oct 1, 1993·Infection and Immunity·P Gueirard, N Guiso
Oct 1, 1993·Infection and Immunity·O G BacaA S Aragon
Mar 1, 1993·Infection and Immunity·A R Melton, A A Weiss

❮ Previous
Next ❯

Citations

Dec 24, 2009·The Journal of Biological Chemistry·Steven McRaeClaudio F Gonzalez
Jan 20, 2000·Infection and Immunity·D H LenzA A Weiss
Feb 26, 2000·Infection and Immunity·C L Weingart, A A Weiss
Dec 19, 2000·Infection and Immunity·V AragonN P Cianciotto
May 12, 2001·FEMS Microbiology Reviews·A M SmithM J Walker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.